Kanematsu T, Higashi H, Takenaka K, Matsumata T, Maehara Y, Sugimachi K
Department of Surgery II, Kyushu University, Fukuoka, Japan.
Hepatogastroenterology. 1990 Dec;37 Suppl 2:160-2.
Changes in the human liver's cellular bio-energy status during warm ischaemia and the subsequent blood reflow period were investigated by means of adenosine triphosphate (ATP) assay. In 8 patients, the average level of ATP in the liver tissue decreased significantly (p less than 0.05) from 28.7 +/- 8.6 mumol/g protein to 19.1 +/- 8.7 mumol/g protein within 10 minutes of warm ischemia. The pattern of decline in ATP levels did not correlate with clinical parameters, including presence of cirrhosis, liver function, type of ischemic method employed and postoperative complications. After reperfusion, ATP levels increased rapidly from 15.1 +/- 8.9 mumol/g protein to 19.6 +/- 8.2 mumol/g protein within 10 minutes, although the difference was not statistically significant. These patients' postoperative course was uneventful, and the results of serum chemistry tests were normal. The present study demonstrates a direct correlation between the levels of tissue ATP and the status of the liver's blood supply.
通过三磷酸腺苷（ATP）检测，研究了人体肝脏在热缺血及随后的血液再灌注期间细胞生物能量状态的变化。在8名患者中，热缺血10分钟内，肝组织中ATP的平均水平从28.7±8.6微摩尔/克蛋白质显著下降（p<0.05）至19.1±8.7微摩尔/克蛋白质。ATP水平的下降模式与临床参数无关，这些参数包括肝硬化的存在、肝功能、采用的缺血方法类型及术后并发症。再灌注后，ATP水平在10分钟内从15.1±8.9微摩尔/克蛋白质迅速升至19.6±8.2微摩尔/克蛋白质，尽管差异无统计学意义。这些患者术后病程平稳，血清化学检测结果正常。本研究表明组织ATP水平与肝脏血液供应状态之间存在直接关联。
