从在乳清中生长的嗜热链球菌生产β-半乳糖苷酶。
Production of beta-galactosidase from Streptococcus thermophilus grown in whey.
作者信息
Rao M V, Dutta S M
出版信息
Appl Environ Microbiol. 1977 Aug;34(2):185-8. doi: 10.1128/aem.34.2.185-188.1977.
Abstract
beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing the enzyme units available in their absence from 660 U/liter of medium to 18,200 and 10,000 U/liter of medium, respectively, in their presence. There was an insignificant increase in the production of enzyme in the presence of added inorganic nitrogen and phosphorus sources. Enzymatic hydrolysis for recuction of lactose content in aqueous solution and in skim milk was studied.
摘要
β-D-半乳糖苷酶（EC 3.2.1.23）是从在脱蛋白奶酪乳清中生长的嗜热链球菌中提取的。酶产量最高时的最佳培养条件为pH 7.0、40℃和24小时。蛋白胨（2.0%，重量/体积）和玉米浆（2.8%，重量/体积）具有很强的刺激作用，在它们存在时，培养基中可利用的酶单位分别从无它们时的660 U/升增加到18200 U/升和10000 U/升。添加无机氮源和磷源时，酶产量的增加不显著。研究了酶法水解以降低水溶液和脱脂乳中的乳糖含量。
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