Nishikawa S, Takagi T, Sasa S
Department of Histology, Kanagawa Dental College, Japan.
J Electron Microsc (Tokyo). 1990;39(5):404-7.
The localization of amelogenin, an enamel matrix protein, was examined by ultrastructural immunocytochemistry using unembedded ultrathin frozen sections of undecalcified rat incisor ameloblasts. Antibody against bovine amelogenin labeled Golgi complexes, secretory granules, and lysosomal structures in the preameloblasts and inner enamel-secretory ameloblasts as well as the enamel. The antibody also labeled dentin matrix facing preameloblasts. These results support the findings in previous reports using conventional epon embedded specimens. However, rough-surfaced endoplasmic reticulum failed to be labeled by this antibody.
利用未脱钙大鼠切牙成釉细胞的超薄冷冻切片，通过超微结构免疫细胞化学方法检测了釉原蛋白（一种釉质基质蛋白）的定位。抗牛釉原蛋白抗体标记了成釉前期细胞和内釉质分泌期成釉细胞中的高尔基体、分泌颗粒和溶酶体结构，以及釉质。该抗体还标记了面向成釉前期细胞的牙本质基质。这些结果支持了以往使用常规环氧树脂包埋标本的报告中的发现。然而，粗面内质网未被该抗体标记。
