Institute of Pathology, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover, Germany.
Ann Hematol. 2011 Mar;90(3):307-13. doi: 10.1007/s00277-010-1072-4. Epub 2010 Sep 15.
The microRNA/miR deregulation in BCR-ABL-negative myelodysplastic-myeloproliferative neoplasms (MDS/MPN) is not known. Myelopoiesis-associated miR-10a, miR-17-5p, miR-155, miR-223 and miR-424 were analysed by real-time polymerase chain reaction (PCR) in bone marrow cells of atypical chronic myeloid leukaemia (aCML, n = 7) and chronic myelomonocytic leukaemia (CMML, n = 8) and were compared to BCR-ABL-positive chronic myelogenous leukaemia (CML, n = 10) and non-neoplastic haematopoiesis (n = 10). Down-regulation of miR-10a was found in CMML but also in CML (each p < 0.05, versus controls). Overexpression of miR-424 was detected in aCML (p < 0.05, versus CML and controls). Despite different compositions of bone marrow cells, expression of myelopoiesis-associated microRNA shows mainly similar patterns in aCML and its main differential diagnosis CMML and does not allow discrimination of these two MDS/MPN entities. Therefore, the link of deregulated microRNA expression to disease-related phenotype and the underlying molecular mechanism are still unknown.
BCR-ABL 阴性骨髓增生异常/骨髓增殖性肿瘤（MDS/MPN）中的 microRNA/miR 失调尚不清楚。通过实时聚合酶链反应（PCR）分析了骨髓细胞中与髓系发生相关的 miR-10a、miR-17-5p、miR-155、miR-223 和 miR-424，比较了非典型慢性髓细胞性白血病（aCML，n=7）和慢性髓单核细胞性白血病（CMML，n=8）与 BCR-ABL 阳性慢性髓细胞性白血病（CML，n=10）和非肿瘤性造血（n=10）。发现 CMML 中 miR-10a 下调，CML 中 miR-10a 也下调（与对照相比，p<0.05）。在 aCML 中检测到 miR-424 的过表达（与 CML 和对照相比，p<0.05）。尽管骨髓细胞组成不同，但与髓系发生相关的 microRNA 的表达主要在 aCML 及其主要鉴别诊断 CMML 中表现出相似的模式，无法区分这两种 MDS/MPN 实体。因此，失调 microRNA 表达与疾病相关表型的联系及其潜在的分子机制仍不清楚。
