埃塞俄比亚耐多药结核病的表型和基因型分析。

Phenotypic and genotypic analysis of multidrug-resistant tuberculosis in Ethiopia.

机构信息

Infectious and Other Diseases Research Department, Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia. macagonafi

出版信息

Int J Tuberc Lung Dis. 2010 Oct;14(10):1259-65.

DOI:

PMID:20843416

Abstract

SETTING

National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia.

OBJECTIVES

To determine the drug susceptibility pattern of Mycobacterium tuberculosis isolates and to genetically characterise multidrug-resistant tuberculosis (MDR-TB) isolates.

DESIGN

A total of 107 M. tuberculosis isolates recovered during the period December 2005-August 2006 were tested for drug susceptibility against streptomycin, isoniazid, rifampicin and ethambutol (SHRE) using the proportion method on Löwenstein-Jensen medium. The MDR-TB isolates were tested against kanamycin, ciprofloxacin, capreomycin, D-cycloserine and ethionamide. Genotyping was performed using spoligotyping.

RESULTS

MDR-TB was observed in one of the 44 new cases (2.3%) and 45/63 previously treated patients (71.4%). Drug susceptibility testing against second-line drugs (SLDs) showed that 26.1% of all MDR-TB isolates were susceptible to all SLDs tested and 73.9% were resistant to one or more classes of SLD. Extensively drug-resistant (XDR) TB was detected in two isolates (4.4%). T3_ETH was the predominant spoligotype, followed by CAS_KILI. In this African setting, no Beijing spoligotype was identified.

CONCLUSION

Both MDR- and XDR-TB are present in Ethiopian patients. MDR-TB was found to be associated with T3 and Central Asian genotypes.

摘要

背景

埃塞俄比亚亚的斯亚贝巴国家结核参考实验室。

目的

确定结核分枝杆菌分离株的药敏模式，并对耐多药结核（MDR-TB）分离株进行基因特征分析。

设计

2005 年 12 月至 2006 年 8 月期间共检测了 107 株结核分枝杆菌分离株，采用 Löwenstein-Jensen 培养基比例法检测对链霉素、异烟肼、利福平、乙胺丁醇（SHRE）的药敏性。MDR-TB 分离株用卡那霉素、环丙沙星、卷曲霉素、D-环丝氨酸和乙硫异烟胺进行检测。基因分型采用 spoligotyping。

结果

44 例新发病例中发现 1 例（2.3%），63 例既往治疗患者中发现 45 例（71.4%）为 MDR-TB。对二线药物（SLD）的药敏试验显示，所有 MDR-TB 分离株中 26.1%对所有测试的 SLD 均敏感，73.9%对一种或多种 SLD 类药物耐药。检测到两例（4.4%）广泛耐药（XDR）结核。T3_ETH 是主要的 spoligotype，其次是 CAS_KILI。在这种非洲环境中，未发现北京 spoligotype。