Kanclerska J, Kanclerski K, Hryniewicz W
Zakład Bakteriologii PZH w Warszawie.
Med Dosw Mikrobiol. 1990;42(3-4):101-7.
Induced mutants of S. agalactiae which differed in surface structures were used for the study. The aim of using them was to try to correlate the presence of defined structures or surface properties with the ability of group B streptococci to attach to epithelial cells. The presence of protein antigen R conditioned strong binding of S. agalactiae cells to hydrophobic gel. Strains bearing clumping factor (CF) showed high surface hydrophobicity and presented compact growth in serum soft agar. However, there was no correlation between high surface hydrophobicity and the ability to adhere. Fibrinogen binding decreased the attachment to epithelial cells of CF-positive strains. Preincubation of bacterial cells with lectin (ConA) did not influence the attachment of S. agalactiae strains with protein surface antigen but increased the adhesion of the strains with polysaccharide antigen or untypable.
使用了表面结构不同的无乳链球菌诱导突变体进行研究。使用它们的目的是试图将特定结构或表面特性的存在与B族链球菌附着于上皮细胞的能力联系起来。蛋白质抗原R的存在使无乳链球菌细胞与疏水凝胶强烈结合。携带聚集因子（CF）的菌株表现出高表面疏水性，并在血清软琼脂中呈现致密生长。然而，高表面疏水性与粘附能力之间没有相关性。纤维蛋白原结合降低了CF阳性菌株对上皮细胞的附着。用凝集素（伴刀豆球蛋白A）对细菌细胞进行预孵育不会影响具有蛋白质表面抗原的无乳链球菌菌株的附着，但会增加具有多糖抗原或不可分型菌株的粘附。
