• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

B族链球菌表面免疫原性蛋白的定位

Localization of surface immunogenic protein on group B streptococcus.

作者信息

Rioux S, Martin D, Ackermann H W, Dumont J, Hamel J, Brodeur B R

机构信息

Unité de Recherche en Vaccinologie, Centre Hospitalier Universitaire de Québec et Université Laval, Ste-Foy, Québec, Canada G1V 4G2.

出版信息

Infect Immun. 2001 Aug;69(8):5162-5. doi: 10.1128/IAI.69.8.5162-5165.2001.

DOI:10.1128/IAI.69.8.5162-5165.2001
PMID:11447199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC98613/
Abstract

The localization and accessibility of the group B streptococcus (GBS) surface immunogenic protein (Sip) at the surface of intact GBS cells were studied by flow cytometric assay and immunogold electron microscopy. Antibodies present in pooled sera collected from mice after immunization with purified recombinant Sip efficiently recognized native Sip at the surfaces of the different GBS strains tested, which included representatives of all nine serotypes. Examination of GBS cells by immunogold electron microscopy revealed that the Sip-specific antibodies attached preferentially to polar sites and the septal region. This result confirmed that Sip is exposed at the intact-cell surface, but it also suggests that its distribution is restricted to certain regions of the cell.

摘要

通过流式细胞术检测和免疫金电子显微镜技术，研究了B族链球菌（GBS）表面免疫原性蛋白（Sip）在完整GBS细胞表面的定位及可及性。用纯化的重组Sip免疫小鼠后，从其收集的混合血清中的抗体能有效识别所检测的不同GBS菌株表面的天然Sip，这些菌株包括所有九种血清型的代表菌株。通过免疫金电子显微镜检查GBS细胞发现，Sip特异性抗体优先附着于极性位点和隔膜区域。这一结果证实Sip暴露于完整细胞表面，但也表明其分布局限于细胞的某些区域。

相似文献

1
Localization of surface immunogenic protein on group B streptococcus.B族链球菌表面免疫原性蛋白的定位
Infect Immun. 2001 Aug;69(8):5162-5. doi: 10.1128/IAI.69.8.5162-5165.2001.
2
Naturally occurring antibodies for the group B streptococcal surface immunogenic protein (Sip) in pregnant women and newborn babies.孕妇和新生儿体内针对B族链球菌表面免疫原性蛋白（Sip）的天然抗体。
Vaccine. 2006 Nov 17;24(47-48):6905-12. doi: 10.1016/j.vaccine.2006.06.020. Epub 2006 Jun 27.
3
Genome analysis reveals pili in Group B Streptococcus.基因组分析揭示B族链球菌中的菌毛。
Science. 2005 Jul 1;309(5731):105. doi: 10.1126/science.1111563.
4
BibA: a novel immunogenic bacterial adhesin contributing to group B Streptococcus survival in human blood.BibA：一种新型免疫原性细菌黏附素，有助于B族链球菌在人体血液中存活。
Mol Microbiol. 2007 Feb;63(3):754-67. doi: 10.1111/j.1365-2958.2006.05555.x. Epub 2007 Jan 8.
5
Immunoelectron microscopic analysis of polysaccharide and protein surface antigens in wild strains of group B streptococci.B族链球菌野生菌株中多糖和蛋白质表面抗原的免疫电子显微镜分析
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1982 Dec;253(3):331-43.
6
Identification of group B streptococcal Sip protein, which elicits cross-protective immunity.
Infect Immun. 2000 Oct;68(10):5610-8. doi: 10.1128/IAI.68.10.5610-5618.2000.
7
Temperature-Induced Self-Assembly of the Group B Streptococcus (GBS) Fusion Antigen GBS-NN.温度诱导的 B 族链球菌（GBS）融合抗原 GBS-NN 的自组装。
Mol Pharm. 2018 Jul 2;15(7):2584-2593. doi: 10.1021/acs.molpharmaceut.8b00101. Epub 2018 May 31.
8
[The role of surface structures of Streptococcus agalactiae in adhesion to epithelial cells].[无乳链球菌表面结构在上皮细胞黏附中的作用]
Med Dosw Mikrobiol. 1990;42(3-4):101-7.
9
Characterization of complex B cell epitopes on woodchuck hepatitis virus surface antigens by using plasmids encoding chimeric proteins and DNA immunization.利用编码嵌合蛋白的质粒和DNA免疫法对土拨鼠肝炎病毒表面抗原上的复杂B细胞表位进行鉴定
Virology. 2002 Mar 15;294(2):342-53. doi: 10.1006/viro.2001.1338.
10
Protection from group B streptococcal infection in neonatal mice by maternal immunization with recombinant Sip protein.通过用重组Sip蛋白对母鼠进行免疫来保护新生小鼠免受B族链球菌感染。
Infect Immun. 2002 Sep;70(9):4897-901. doi: 10.1128/IAI.70.9.4897-4901.2002.

引用本文的文献

1
A CRISPRi library screen in group B identifies surface immunogenic protein (Sip) as a mediator of multiple host interactions.在B组中进行的CRISPR干扰文库筛选确定表面免疫原性蛋白（Sip）是多种宿主相互作用的介质。
Infect Immun. 2025 Apr 8;93(4):e0057324. doi: 10.1128/iai.00573-24. Epub 2025 Mar 21.
2
A CRISPRi Library Screen in Group B Identifies Surface Immunogenic Protein (Sip) as a Mediator of Multiple Host Interactions.B组中的CRISPRi文库筛选确定表面免疫原性蛋白（Sip）是多种宿主相互作用的介质。
bioRxiv. 2024 Dec 7:2024.12.06.627252. doi: 10.1101/2024.12.06.627252.
3
Using Surface Immunogenic Protein as a Carrier Protein to Elicit Protective Antibody to Multiple Serotypes for Candidate Group B Streptococcal Glycan Conjugate Vaccines.使用表面免疫原性蛋白作为载体蛋白，针对B族链球菌聚糖结合疫苗候选物引发针对多种血清型的保护性抗体。
Vaccines (Basel). 2024 May 24;12(6):573. doi: 10.3390/vaccines12060573.
4
Development and performance evaluation of a qPCR-based assay for the fully automated detection of group B (GBS) on the Panther Fusion Open Access system.基于 qPCR 的全自动检测 B 群（GBS）的方法的开发和性能评估，该方法在 Panther Fusion Open Access 系统上实现。
Microbiol Spectr. 2024 Jun 4;12(6):e0005724. doi: 10.1128/spectrum.00057-24. Epub 2024 Apr 29.
5
Protein-Based Adjuvants for Vaccines as Immunomodulators of the Innate and Adaptive Immune Response: Current Knowledge, Challenges, and Future Opportunities.作为天然免疫和适应性免疫反应免疫调节剂的疫苗蛋白佐剂：当前认知、挑战与未来机遇
Pharmaceutics. 2022 Aug 11;14(8):1671. doi: 10.3390/pharmaceutics14081671.
6
Research Advances on Tilapia Streptococcosis.罗非鱼链球菌病的研究进展
Pathogens. 2021 May 6;10(5):558. doi: 10.3390/pathogens10050558.
7
Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women.实时 PCR 检测孕妇中无乳链球菌的开发和分析验证。
BMC Pregnancy Childbirth. 2020 Jun 9;20(1):352. doi: 10.1186/s12884-020-03038-z.
8
Mucosal Vaccination with -Secreting Surface Immunological Protein Induces Humoral and Cellular Immune Protection against Group B in a Murine Model.用分泌表面免疫蛋白的粘膜疫苗接种在小鼠模型中诱导针对B组的体液免疫和细胞免疫保护。
Vaccines (Basel). 2020 Mar 26;8(2):146. doi: 10.3390/vaccines8020146.
9
Status of group B streptococcal vaccine development.B族链球菌疫苗的研发现状。
Clin Exp Vaccine Res. 2018 Jan;7(1):76-81. doi: 10.7774/cevr.2018.7.1.76. Epub 2018 Jan 29.
10
Vaccination With a Latch Peptide Provides Serotype-Independent Protection Against Group B Streptococcus Infection in Mice. latch 肽疫苗接种为小鼠提供针对 B 族链球菌感染的血清型非依赖性保护。
J Infect Dis. 2017 Dec 27;217(1):93-102. doi: 10.1093/infdis/jix565.

本文引用的文献

1
Identification of group B streptococcal Sip protein, which elicits cross-protective immunity.
Infect Immun. 2000 Oct;68(10):5610-8. doi: 10.1128/IAI.68.10.5610-5618.2000.
2
Protection against experimental infection with group B streptococcus by immunization with a bivalent protein vaccine.用二价蛋白疫苗免疫预防B族链球菌实验性感染
Vaccine. 1999 Feb 5;17(5):454-8. doi: 10.1016/s0264-410x(98)00218-7.
3
Epidemiology of group B streptococcal disease in the United States: shifting paradigms.美国B族链球菌病的流行病学：不断变化的模式
Clin Microbiol Rev. 1998 Jul;11(3):497-513. doi: 10.1128/CMR.11.3.497.
4
Surface protein expression in group B streptococcal invasive isolates.B族链球菌侵袭性分离株的表面蛋白表达
Adv Exp Med Biol. 1997;418:635-7. doi: 10.1007/978-1-4899-1825-3_148.
5
Immune response to type III group B streptococcal polysaccharide-tetanus toxoid conjugate vaccine.对III型B族链球菌多糖-破伤风类毒素结合疫苗的免疫反应。
J Clin Invest. 1996 Nov 15;98(10):2308-14. doi: 10.1172/JCI119042.
6
Structural and immunochemical characterization of the type VIII group B Streptococcus capsular polysaccharide.B族链球菌VIII型荚膜多糖的结构与免疫化学特性
J Biol Chem. 1996 Apr 12;271(15):8786-90. doi: 10.1074/jbc.271.15.8786.
7
Invasive group B streptococcal disease: the emergence of serotype V.侵袭性B族链球菌病：血清型V的出现。
J Infect Dis. 1996 Feb;173(2):365-73. doi: 10.1093/infdis/173.2.365.
8
Protein rib: a novel group B streptococcal cell surface protein that confers protective immunity and is expressed by most strains causing invasive infections.蛋白质核糖：一种新型B族链球菌细胞表面蛋白，可提供保护性免疫，且大多数引起侵袭性感染的菌株都可表达该蛋白。
J Exp Med. 1993 Jun 1;177(6):1593-603. doi: 10.1084/jem.177.6.1593.
9
Stimulation of protective antibodies against type Ia and Ib group B streptococci by a type Ia polysaccharide-tetanus toxoid conjugate vaccine.I a型多糖-破伤风类毒素结合疫苗对I a型和I b型B族链球菌保护性抗体的刺激作用。
Infect Immun. 1993 Nov;61(11):4760-6. doi: 10.1128/iai.61.11.4760-4766.1993.
10
Adherence of group B streptococci to cultured epithelial cells: roles of environmental factors and bacterial surface components.B族链球菌对培养上皮细胞的黏附：环境因素和细菌表面成分的作用。
Infect Immun. 1994 Jun;62(6):2450-8. doi: 10.1128/iai.62.6.2450-2458.1994.