Department of Pharmacy and Pharmacology, State University of Maringá, Maringá, PR, Brazil.
Planta Med. 2011 Feb;77(3):236-41. doi: 10.1055/s-0030-1250315. Epub 2010 Sep 15.
The objective of this study was to investigate the effects of chronic administration of a semi-purified extract (Purified Extract A--PEA; 4, 8, or 16 mg/kg) of PAULLINIA CUPANA (guaraná) seeds on rats submitted to the elevated T-maze (ETM) model of generalized anxiety and panic disorders. The selective serotonin (5-HT) reuptake inhibitor (SSRI) paroxetine (PAR; 3 mg/kg), was used as a positive control. To evaluate possible serotonergic and dopaminergic neurotransmission involvement in the action of PEA during the ETM test, ineffective doses of metergoline (MET; 5-HT (2A/2C) antagonist receptor) or sulpiride (SUL; dopaminergic receptor antagonist) were acutely administered together with the PEA. The locomotion of the rats was assessed in a circular arena following each drug treatment. Both PEA (8 and 16 mg/kg) and PAR (3 mg/kg) increased one-way escape latencies from the open arm of the ETM, indicating a panicolytic effect compared to the control group. MET, in higher doses (1, 2 or 3 mg/kg), produced a panicolytic effect in the ETM test, whereas SUL did not (10, 20 or 40 mg/kg). The panicolytic effect produced by PEA (8 mg/kg) was blocked by both MET (2 mg/kg) and SUL (20 mg/kg), whereas the panicolytic effect produced by PAR (3 mg/kg) was blocked only by MET (2 mg/kg). These results show that chronic treatment with PEA produces a panicolytic effect during the ETM test, and that the dopaminergic and the serotonergic neurotransmission systems are involved in this effect.
本研究的目的是探讨慢性给予 PAULLINIA CUPANA（瓜拉那）种子半纯化提取物（Purified Extract A--PEA；4、8 或 16mg/kg）对高架十字迷宫（ETM）模型中广泛性焦虑和惊恐障碍大鼠的影响。选择性 5-羟色胺（5-HT）再摄取抑制剂（SSRI）帕罗西汀（PAR；3mg/kg）被用作阳性对照。为了评估在 ETM 测试中 PEA 作用可能涉及的 5-HT 和多巴胺能神经递质传递，给予无效剂量的麦角乙脲（MET；5-HT（2A/2C）拮抗剂受体）或舒必利（SUL；多巴胺能受体拮抗剂）与 PEA 一起急性给药。在每次药物处理后，通过圆形竞技场评估大鼠的运动。PEA（8 和 16mg/kg）和 PAR（3mg/kg）均增加了大鼠从 ETM 开臂的单向逃避潜伏期，表明与对照组相比具有抗惊恐作用。MET（1、2 或 3mg/kg）高剂量产生 ETM 测试中的抗惊恐作用，而 SUL（10、20 或 40mg/kg）则没有。PEA（8mg/kg）产生的抗惊恐作用被 MET（2mg/kg）和 SUL（20mg/kg）阻断，而 PAR（3mg/kg）产生的抗惊恐作用仅被 MET（2mg/kg）阻断。这些结果表明，慢性给予 PEA 在 ETM 测试中产生抗惊恐作用，并且多巴胺能和 5-HT 能神经递质传递系统参与了这种作用。
