Golubovskaia V M, Aprelikova O N, Tomilin N V
Mol Gen Mikrobiol Virusol. 1990 Dec(12):10-2.
The mismatch correction has been studied in human cells and presented in this paper. In the study the experimental model with half-containing hetroduplex (-1 residue in the polylinker region of lac gene) M13 DNA has been used. M 13 DNA was isolated from human cells 24 hours after transfection and transformed into ung+ and ung- cells of Escherichia coli. The percentage of lac- colonies (formed due to frameshift mutation in the lac gene) was analyzed. The increased percent of lac- mutations after human transfection indicated that DNA-uracil can polarize mismatch correction in human cells.
错配修复已在人类细胞中进行了研究，并在本文中呈现。在该研究中，使用了含有半杂合双链体（lac基因多克隆位点区域有-1个残基）的M13 DNA实验模型。转染24小时后从人类细胞中分离出M13 DNA，并将其转化到大肠杆菌的ung+和ung-细胞中。分析了lac-菌落（由于lac基因移码突变形成）的百分比。人类转染后lac-突变百分比增加表明DNA尿嘧啶可使人类细胞中的错配修复极化。
