Niedworok E, Muc-Wierzgon M, Nowakowska-Zajdel E
J Biol Regul Homeost Agents. 2010 Jul-Sep;24(3):377-80.
The aim of the study is to analyse the changes in the profile of fatty acids and their esters in rat hepatocytes that were incubated for 5 hours with different concentrations of MgCl2 (2 and 4mM) in hepatocyte culture medium. The methyl esters of fatty acids were identified with a GC-MS system included in the Hewlett?Packard quadrupolar mass spectrometer, coupled with a Hewlett-Pacard 5890 gas chromatograph with an ionisation potential of 70 eV and recorded on a Vectra 386 computer. We observed differences in the amount of saturated, monounsaturated and polyunsaturated fatty acids among the examined samples. In the control sample, the largest component consisted of the pool of saturated and monounsaturated fatty acids. Analysing the changes in the profile of ester-bound fatty acids, we found statistically significant differences when 4 mM MgCl2 was presented. The amount of C18:2, C18:1b and C20:4a decreased in comparison with the control sample.
本研究的目的是分析在肝细胞培养基中用不同浓度的MgCl2（2和4mM）孵育5小时的大鼠肝细胞中脂肪酸及其酯类的变化情况。脂肪酸甲酯是用惠普四极质谱仪中的气相色谱-质谱联用系统进行鉴定的，该系统与电离电位为70eV的惠普5890气相色谱仪联用，并记录在Vectra 386计算机上。我们观察到在所检测的样本中饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸的含量存在差异。在对照样本中，最大的成分是饱和脂肪酸和单不饱和脂肪酸库。分析酯结合脂肪酸的变化情况时，我们发现当存在4mM MgCl2时具有统计学显著差异。与对照样本相比，C18:2、C18:1b和C20:4a的含量下降。
