College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
Mol Biol Rep. 2011 Mar;38(3):2007-14. doi: 10.1007/s11033-010-0323-8. Epub 2010 Sep 17.
In order to screen candidate genes related to tenderness trait in Qinchuan cattle, we investigated the gene expression profile of Longuissimus dorsi muscle (LDM) tissue and screened differentially expressed genes in LDM from both male and female Qinchuan cattle at 36 months of age utilising Bovine Genome Array. Significance Analysis of Microarrays (SAM) was used to identify the differentially expressed genes, Go (Gene Ontology) and pathways analysis were conducted on which by a free web-based Molecular Annotation System 2.0 (MAS 2.0). Approximately 11,000 probe sets representing 10,000 genes were detected in LDM of 36 month old Qinchuan cattle. After SAM analysis of the microarray data, 598 genes were shown to be differentially expressed. These genes were predominantly involved in cell adhesion, collagen fibril organization and synthesis, immune responses and cell-matrix adhesion. They included cell adhesion molecules (CAMs) and ECM-receptor interaction molecules. Real-time PCR was performed to validate nine of the differentially expressed genes identified by microarray. The results suggest that at the transcriptional level the residual hardness caused by connective tissues, stroma protein and muscle tissues could mainly result in tenderness differences between male and female Qinchuan cattle.
为了筛选秦川牛嫩度性状相关的候选基因，我们利用牛基因组芯片研究了 36 月龄秦川牛公、母牛背最长肌（LDM）组织的基因表达谱，并筛选了 LDM 中的差异表达基因。采用差异倍数法和显著性分析（SAM）筛选差异表达基因，通过免费的在线分子注释系统 2.0（MAS 2.0）进行基因本体论（GO）和通路分析。在 36 月龄秦川牛 LDM 中检测到约 11000 个代表 10000 个基因的探针集。对微阵列数据进行 SAM 分析后，发现有 598 个基因差异表达。这些基因主要参与细胞黏附、胶原纤维组织和合成、免疫反应和细胞基质黏附，包括细胞黏附分子（CAMs）和细胞外基质受体相互作用分子。通过实时 PCR 验证了微阵列鉴定的 9 个差异表达基因。结果表明，在转录水平上，结缔组织、基质蛋白和肌肉组织的残余硬度可能主要导致公、母牛秦川牛嫩度的差异。
