College of Animal Science and Technology, Northwest A & F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, People's Republic of China.
Mol Biol Rep. 2011 Jan;38(1):131-7. doi: 10.1007/s11033-010-0086-2. Epub 2010 Mar 21.
The peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear hormone receptor that regulates adipogenesis and many other biological processes. In the present study, we carried out PCR-SSCP and DNA sequencing analyses to examine SNPs in coding region of the PPARγ gene. A total of 660 individuals from five Chinese cattle breeds were genotyped. We identified three SNPs and their associations with meat quality traits were analyzed in 108 Qinchuan cattle. Two missense mutations and one synonymous mutation were found: 200 A>G (genotypes AA, AB and BB) resulting in D7G change, the silent substitution 42895 C>T (genotypes JJ and JI) and 72472 G>T (genotypes CC, DC and DD) producing Q448H change, respectively. The frequencies of PPARγ-A allele were 0.86, 0.83, 0.80, 0.72 and 0.87 for Qinchuan, Nanyang, Jiaxian, Luxi and Xianan populations, respectively. The frequencies of PPARγ-J allele varied from 0.87 to 0.96 in the five populations. In the 72472 G>T locus, the frequencies of PPARγ-C allele were higher than PPARγ-D allele in the five populations, and ranged from 0.58 to 0.82. Least squares analysis revealed that in 42895 C>T locus, there was a significant effect on tenderness in 18-20 months Qinchuan cattle (P<0.01), and in the 72472 G>T locus, animals with the genotype DC had lower mean values than these with genotype CC (P<0.01) for back fat thickness in 18-20 months, and animals with the genotype DD had lower mean values than these with genotypes CC and DC (P<0.01) for water holding capacity in 21-24 months (P<0.01). The SNPs we have identified may contribute to establishing a more efficient selection program for improving of genetic characteristics in indigenous Chinese cattle.
过氧化物酶体增殖物激活受体 γ（PPARγ）是一种核激素受体，可调节脂肪生成和许多其他生物学过程。在本研究中，我们进行了 PCR-SSCP 和 DNA 测序分析，以检查 PPARγ 基因编码区的 SNPs。从五个中国牛品种中抽取了 660 个人进行基因分型。我们在 108 头秦川牛中鉴定了三个 SNPs，并分析了它们与肉质性状的关联。发现了两个错义突变和一个同义突变：200 A>G（基因型 AA、AB 和 BB）导致 D7G 变化，沉默取代 42895 C>T（基因型 JJ 和 JI）和 72472 G>T（基因型 CC、DC 和 DD）分别产生 Q448H 变化。PPARγ-A 等位基因的频率分别为 0.86、0.83、0.80、0.72 和 0.87，用于秦川、南阳、贾县、鲁西和仙安种群。PPARγ-J 等位基因的频率在五个种群中从 0.87 到 0.96 不等。在 72472 G>T 位点，五个种群中 PPARγ-C 等位基因的频率均高于 PPARγ-D 等位基因，范围为 0.58 至 0.82。最小二乘法分析表明，在 42895 C>T 位点，18-20 个月的秦川牛中，该位点对嫩度有显著影响（P<0.01），在 72472 G>T 位点，基因型 DC 的动物的背脂厚度平均值低于基因型 CC（P<0.01），21-24 个月的基因型 DD 的动物的背脂厚度平均值低于基因型 CC 和 DC（P<0.01）的动物的背脂厚度平均值低于基因型 CC 和 DC（P<0.01）水保持能力（P<0.01）。我们鉴定的这些 SNPs 可能有助于建立一个更有效的选择计划，以提高中国本土牛的遗传特性。
