Peng Fang, Li Jin-Mei, Li Qiong, Yao Zhi-Ping, Zhou Dong
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Jul;41(4):590-4.
To investigate the different expression of GluR5 and GluR6 in rat astrocyte induced with Coriaria Lactone (CL) and ATPA and to discuss the possible mechanism of the inducing epileptic model.
Astrocyte of neonate rats was treated with CL and ATPA respectively and the astrocyte treated with normal saline as control. The mRNA and protein levels of GluR5 and GluR6 were detected by RT-PCR and western blotting.
Compared with the control, the expression of GluR5 was increased significantly in the astrocyte induced by ATPA (P < 0.05), however, there was no alteration of GluR6 expression. The expression of GluR5 and GluR6 were decreased significantly in CL-treated group (P < 0.05).
The present study showed ATPA up-regulated mRNA and protein levels of GluR5, but coriaria lactone down-regulated the GluR5 and GluR6 of astrocyte. The results indicate that there are different pathologic mechanisms of GluR5 and GluR6 in different neurotoxicants inducing model of epilepsy. The molecular mechanism need to be studied further in the future.
研究马桑内酯（CL）和ATPA诱导的大鼠星形胶质细胞中GluR5和GluR6的表达差异，并探讨诱导癫痫模型的可能机制。
分别用CL和ATPA处理新生大鼠星形胶质细胞，以生理盐水处理的星形胶质细胞作为对照。采用RT-PCR和蛋白质印迹法检测GluR5和GluR6的mRNA和蛋白质水平。
与对照组相比，ATPA诱导的星形胶质细胞中GluR5表达显著增加（P<0.05），而GluR6表达无变化。CL处理组中GluR5和GluR6的表达显著降低（P<0.05）。
本研究表明ATPA上调了GluR5的mRNA和蛋白质水平，但马桑内酯下调了星形胶质细胞中GluR5和GluR6的表达。结果表明，在不同神经毒物诱导的癫痫模型中，GluR5和GluR6存在不同的病理机制。其分子机制有待进一步研究。
