[蛋白激酶C在钙离子载体A23187诱导HL-60细胞向树突状细胞分化过程中的作用]

[The role of protein kinase C in the process of HL-60 cells induced into dendritic cells by calcium ionophore A23187].

作者信息

Yang Kun, Li Qiang

机构信息

Department of Pediatrics, Teaching Hospital of Chengdu University of TCM, Chengdu 610075, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Jul;41(4):630-3.

PMID:20848784

Abstract

OBJECTIVE

To Investigate the role of protein kinase C (PKC) signal transduction in the process of HL-60 leukemic cells induced into dendritic cells (DCs) by calcium ionophore A23187.

METHODS

HL-60 cells were pretreated with protein kinase C (PKC) inhibitor Bis-1 for 24 hours followed by cultured with A23187 for another 36 hours, and the morphology, immunophenotype and function of stimulating proliferation of allogeneic T cells were compared with the cells only treated with A23187.

RESULTS

The morphological changes, surface marker expressions and ability to stimulating the proliferation of allogeneic T cells were inhibited in DCs derived from HL-60 cells treated with PKC inhibitor Bis-1. The percentage of CD83, CD80, CD86 expressing on DCs induced by A23187 significantly decreased to (28.97 +/- 4.05)% vs. (13.23 +/- 2.15)%, (19.10 +/- 5.46)% vs. (9.70 +/- 1.69)%, (41.03 +/- 6.43)% vs. (23.37 +/- 7.50)%, respectively (P < 0.05).

CONCLUSION

The induction of HL-60 cells induced into DCs by A23187 can be inhibited by PKC inhibitor Bis-1, this suggested that PKC signal transduction pathway might be involved in the process of HL-60 leukemic cells differentiated into DCs by A23187 induction.

摘要

目的

探讨蛋白激酶C（PKC）信号转导在钙离子载体A23187诱导HL-60白血病细胞分化为树突状细胞（DCs）过程中的作用。

方法

用蛋白激酶C（PKC）抑制剂Bis-1预处理HL-60细胞24小时，再用A23187培养36小时，将其形态、免疫表型及刺激同种异体T细胞增殖的功能与仅用A23187处理的细胞进行比较。

结果

用PKC抑制剂Bis-1处理的HL-60细胞来源的DCs，其形态变化、表面标志物表达及刺激同种异体T细胞增殖的能力均受到抑制。A23187诱导的DCs上CD83、CD80、CD86的表达百分比显著降低，分别为（28.97±4.05）%对（13.23±2.15）%、（19.10±5.46）%对（9.70±1.69）%、（41.03±6.43）%对（23.37±7.50）%（P<0.05）。