Li Bo-Wen, Liang Sha, Xie Jin-Xin, Li Jie, Lan Bei, Li Yong-Qing, Li Hui-Ying, Cheng Wei-Xia, Li Dan-Di, Jin Yu, Duan Zhao-Jun
First Clinical Medical College of Lanzhou University, Lanzhou 730000, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Feb;24(1):11-3.
To identification and analysis Aichi virus from diarrhea and normal children in Lanzhou, and discuss the relationship between Aichi virus and Infant Diarrhea.
According to the literature published data, Using RT-PCR method to amplified Aichi virus 3CD fragment and the positive products were sequenced and determined, and made the alignment analysis between the nucleotide sequences of the amplified fragment with the known sequence of this virus.
There was 1 case detection of Aichi virus in the 46 hospitalized children with diarrhea and 299 children with diarrhea out-patients specifically, Overall detection rate was 0.06%, and there was no Aichi virus was detected in normal control children. 2 viral 3CD gene and the known reference strains of nucleotide sequences were 97%, while phylogenetic analysis showed that genotype of 2 viral belongs to the B.
There existed B Genotype of Aichi virus in China, and more research is needed to clarified the etiology and epidemiology of Aichi virus characteristics.
对兰州地区腹泻儿童和正常儿童进行爱知病毒的鉴定与分析，探讨爱知病毒与婴幼儿腹泻的关系。
根据文献公布的数据，采用RT-PCR方法扩增爱知病毒3CD片段，对阳性产物进行测序及序列测定，并将扩增片段的核苷酸序列与该病毒已知序列进行比对分析。
在46例住院腹泻儿童及299例门诊腹泻儿童中，共检测出1例爱知病毒，总检出率为0.06%，正常对照儿童中未检测到爱知病毒。2株病毒3CD基因与已知参考毒株核苷酸序列同源性为97%，系统进化分析表明2株病毒基因型均属于B型。
中国存在爱知病毒B基因型，尚需进一步研究明确爱知病毒的病原学及流行病学特征。
