[高迁移率族蛋白B1激活牙周膜成纤维细胞表达细胞因子的作用]
[Effects of high mobility group box 1 in activating periodontal ligament fibroblasts to express cytokine].
作者信息
Sun Qin-feng, Xu Yan, Song Hui, Hu Ying-wei, Yang Pi-shan
机构信息
Dept. of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Aug;28(4):443-6.
OBJECTIVE
To investigate the influence of high mobility group box 1 (HMGB1) on the expression of interleukin 6 (IL-6), receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) on periodontal ligament fibroblasts.
METHODS
Human periodontal ligament fibroblasts were stimulated with HMGB1 at concentrations of 10, 30, and 100 ng x mL(-1) for 24 h. RT-PCR and Western blot analysis were performed to check mRNA and protein expression of IL-6, RANKL and OPG on the cells.
RESULTS
The ratio of RANKL/OPG was increased at both mRNA and protein level after HMGB1 stimulation at 10, 30, 100 ng x mL(-1). Inflammatory cytokine IL-6 was upregulated by HMGB1 at the concentration of 100 ng x mL(-1).
CONCLUSION
Increased ratio of RANKL/OPG and IL-6 on periodontal ligament fibroblasts suggests that HMGB1 might play a role in the pathogenesis and progression of periodontal disease.
目的
探讨高迁移率族蛋白B1（HMGB1）对牙周膜成纤维细胞白细胞介素6（IL-6）、核因子κB受体活化因子配体（RANKL）及骨保护素（OPG）表达的影响。
方法
用浓度为10、30和100 ng·mL⁻¹的HMGB1刺激人牙周膜成纤维细胞24小时。采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测细胞中IL-6、RANKL和OPG的mRNA及蛋白表达。
结果
在10、30、100 ng·mL⁻¹的HMGB1刺激后，RANKL/OPG比值在mRNA和蛋白水平均升高。炎症细胞因子IL-6在100 ng·mL⁻¹浓度的HMGB1刺激下上调。
结论
牙周膜成纤维细胞中RANKL/OPG比值及IL-6升高提示HMGB1可能在牙周疾病的发病机制及进展中起作用。
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