qPCR 检测法用于定量与微生物高氯酸盐还原相关的基因和基因表达。
qPCR assays to quantify genes and gene expression associated with microbial perchlorate reduction.
机构信息
The University of Texas at Austin, Department of Civil, Architectural, and Environmental Engineering, 1 University Station C1786, Austin, TX 78712, United States.
出版信息
J Microbiol Methods. 2010 Nov;83(2):270-4. doi: 10.1016/j.mimet.2010.09.002. Epub 2010 Sep 16.
PMID:20849890
Abstract
Quantitative PCR (qPCR) assays targeting cld (developed in this work) and pcrA (previously described) were used to quantify these perchlorate-related genes in a perchlorate-reducing enrichment culture. Transcript copies were quantified in perchlorate-reducing Rhodocyclaceae strain JDS4. Oxygen and nitrate inhibited expression of cld and pcrA.
摘要
本工作开发的针对 cld 的定量 PCR(qPCR)检测法和先前描述的 pcrA 检测法被用于定量检测一株还原氯酸盐的富集培养物中的这些与氯酸盐相关的基因。在还原氯酸盐的红环菌科菌株 JDS4 中定量了转录物拷贝数。氧和硝酸盐抑制 cld 和 pcrA 的表达。
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