University of Toledo Health Sciences Campus, Toledo, Ohio, USA.
J Urol. 2010 Nov;184(5):2192-6. doi: 10.1016/j.juro.2010.06.092. Epub 2010 Sep 20.
We evaluated the comparative effects of intraprostatic injection of cobra cardiotoxin D and botulinum toxin type A on prostate structure in the rat model.
A total of 18 Sprague-Dawley® rats weighing 500 to 600 gm received a single 0.1 ml injection of saline (6), botulinum toxin type A (6) or the cardiotoxin D (6) component of cobra (Naja naja atra) toxin in the right and left ventral lobes of the prostate. At 14 days the rats were sacrificed. The prostate glands were harvested, weighed and processed for immunohistochemical and morphological studies.
Prostate glands injected with cardiotoxin D showed significantly decreased weight compared to that of prostates injected with botulinum toxin type A and the saline control. Prostatic atrophy in the glandular component with flattening of the epithelial lining was seen histologically in rats that received botulinum toxin and cardiotoxin D. Each group injected with cardiotoxin D and botulinum toxin showed a significant increase in the number of apoptotic cells compared with controls while only the botulinum toxin group showed a significant increase in the number of proliferating cells. Only rats injected with botulinum toxin had body weight loss.
Our study shows that intraprostatic injection of cobra cardiotoxin D induces prostatic atrophy and leads to a decrease in prostatic weight greater than that of intraprostatic injection of botulinum toxin type A. No systemic effects, such as decreased body weight, were noted after cardiotoxin D injection. Further studies are warranted but the statistically significant decrease in the number of proliferating cells implies a prolonged effect of cardiotoxin D.
我们评估了蛇毒细胞毒素 D 和肉毒毒素 A 对前列腺结构的比较作用。
18 只体重 500 至 600 克的 Sprague-Dawley®大鼠，每只接受 0.1ml 生理盐水（6 只）、肉毒毒素 A（6 只）或眼镜蛇（Naja naja atra）毒素的细胞毒素 D（6 只）成分在前列腺的左右腹叶中的单一注射。14 天后，处死大鼠。采集前列腺，称重并进行免疫组织化学和形态学研究。
与肉毒毒素 A 注射组和生理盐水对照组相比，注射细胞毒素 D 的前列腺重量明显降低。接受肉毒毒素和细胞毒素 D 注射的大鼠在组织学上显示出腺体成分的前列腺萎缩，上皮衬里变平。与对照组相比，注射细胞毒素 D 和肉毒毒素的每组均显示出凋亡细胞数量的显著增加，而只有肉毒毒素组显示出增殖细胞数量的显著增加。只有注射肉毒毒素的大鼠体重减轻。
我们的研究表明，前列腺内注射眼镜蛇细胞毒素 D 可引起前列腺萎缩，并导致前列腺重量下降大于前列腺内注射肉毒毒素 A。注射细胞毒素 D 后没有观察到全身效应，如体重下降。需要进一步研究，但增殖细胞数量的统计学显著减少意味着细胞毒素 D 的长期作用。
