评估伊朗东南部输血后隐匿性乙型肝炎病毒感染患者的 CXCL12（SDF-1α）多态性及其血清水平。

Assessment of CXCL12 (SDF-1α) polymorphisms and its serum level in posttransfusion occult HBV-infected patients in Southeastern Iran.

机构信息

Molecular Medicine Research Center, Rafsanjan, Iran.

出版信息

Arch Med Res. 2010 Jul;41(5):338-42. doi: 10.1016/j.arcmed.2010.07.001.

DOI:10.1016/j.arcmed.2010.07.001

PMID:20851290

Abstract

BACKGROUND

Occult hepatitis B infection (OBI) is defined as a form of hepatitis in which, despite absence of detectable HBsAg, HBV-DNA is present in peripheral blood of patients. The main aim of this study was to determine an association between polymorphisms in +801 of CXCL12 (SDF-1α) and its serum level in OBI patients.

METHODS

In this experimental study, plasma samples of 3700 blood donors were tested for HBsAg and anti-HBc by ELISA. The HBsAg(-)/anti-HBc(+) samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples assigned as OBI cases and PCR-RFLP techniques were performed to examine the CXCL12 (SDF-1α) polymorphisms. The serum level of CXCL12 (SDF-1α) was also analyzed by ELISA.

RESULTS

Of 3700 blood samples, 352 (9.5%) were HBsAg/anti-HBc(+) and HBV-DNA was detected in 57/352 (16.1%) of HBsAg(-)/anti-HBc(+) samples. Our results showed a significant difference in genotypes and alleles of +801 region of CXCL12 (SDF-1α). However, the serum level of CXCL12 (SDF-1α) was decreased in OBI patients but was not significant. Our results also showed that the alleles of +801 region of CXCL12 (SDF-1α) were also not associated with serum level of the chemokine.

CONCLUSIONS

The polymorphisms in +801 region of CXCL12 (SDF-1α) are possibly related to OBI.

摘要

背景

隐匿性乙型肝炎感染（OBI）定义为一种形式的肝炎，尽管无法检测到 HBsAg，但患者外周血中存在 HBV-DNA。本研究的主要目的是确定 CXCL12（SDF-1α）+801 多态性与其在 OBI 患者中的血清水平之间的关联。

方法

在这项实验研究中，通过 ELISA 检测 3700 份献血者的血浆样本中的 HBsAg 和抗-HBc。选择 HBsAg（-）/抗-HBc（+）样本，并通过 PCR 筛选 HBV-DNA。将 HBV-DNA 阳性样本指定为 OBI 病例，并进行 PCR-RFLP 技术以检查 CXCL12（SDF-1α）多态性。还通过 ELISA 分析 CXCL12（SDF-1α）的血清水平。

结果

在 3700 份血样中，有 352 份（9.5%）为 HBsAg/抗-HBc（+），在 57/352 份（16.1%）HBsAg（-）/抗-HBc（+）样本中检测到 HBV-DNA。我们的结果显示 CXCL12（SDF-1α）+801 区的基因型和等位基因存在显著差异。然而，OBI 患者的 CXCL12（SDF-1α）血清水平降低，但无统计学意义。我们的结果还表明，CXCL12（SDF-1α）+801 区的等位基因也与趋化因子的血清水平无关。