Marusić A, Djikić I, Marusić M
Department of Anatomy Zagreb University School of Medicine, Yugoslavia.
Agents Actions. 1990 Nov;31(3-4):280-4. doi: 10.1007/BF01997620.
We investigated the effects of a single i.v. injection of recombinant human interleukin 1 alpha (IL-1 alpha) on the morphology and the cellularity of several lymphoid organs in normal mice. The injection of 100 U of IL-1 alpha resulted in maximal neutrophilia and leukocytosis at 1 h. By 72 h, the numbers of mononuclears, but not that of polymorphonuclears, returned to baseline levels. Absolute increase in mononuclears was paralleled by relative lymphopenia. Changes in the peripheral blood coincided with rapid decrease in the spleen cellularity and white pulp volume (especially the marginal zone), and an increase in the red pulp volume. Bone marrow cellularity was increased at 1 h, but returned to control levels by 6 h after IL-1 injection. Thymus cell depletion and cortex atrophy were maximal at 6 h and could be observed throughout the experiment. These findings indicate that leukocytosis induced by a single i.v. injection of IL-1 alpha in normal mice is concomitant with a rapid cell depletion of the spleen and thymus. Morphological and cellular changes in lymphoid organs may represent the mobilization of immunocompetent cells during the development of the inflammatory response.
我们研究了单次静脉注射重组人白细胞介素1α（IL-1α）对正常小鼠多个淋巴器官的形态和细胞数量的影响。注射100 U的IL-1α在1小时时导致最大程度的中性粒细胞增多和白细胞增多。到72小时时，单核细胞数量恢复到基线水平，而多形核细胞数量未恢复。单核细胞的绝对增加与相对淋巴细胞减少同时出现。外周血的变化与脾脏细胞数量和白髓体积（尤其是边缘区）迅速减少以及红髓体积增加相吻合。骨髓细胞数量在1小时时增加，但在注射IL-1后6小时恢复到对照水平。胸腺细胞耗竭和皮质萎缩在6小时时最为严重，且在整个实验过程中均可观察到。这些发现表明，正常小鼠单次静脉注射IL-1α诱导的白细胞增多与脾脏和胸腺的快速细胞耗竭同时发生。淋巴器官的形态和细胞变化可能代表炎症反应发展过程中免疫活性细胞的动员。
