氧气与可溶性细胞色素氧化酶反应的低温动力学
Low-temperature kinetics of the reaction of oxygen and solubilized cytochrome oxidase.
作者信息
Chance B, Saronio C, Leigh J S, Ingledew W J, King T E
出版信息
Biochem J. 1978 Jun 1;171(3):787-98. doi: 10.1042/bj1710787.
Abstract
The reaction of solubilized cytochrome oxidase in the fully reduced state with O2 at low temperatures reveals components with characteristics similar to those observed with the membrane-bound oxidase, namely compounds A and B, which are proposed to be 'oxy' and 'peroxy' compounds respectively. Similar species are identified in both solubilized and membrane-bound oxidases; the reaction velocity constant for the reation with O2 and the dissociation constant are decreased 2-3-fold in the solubilied preparation as compared with the membrane-bound species, owing to decreased reactivity towards O2 in the former. The oxidase prepared in the mixed-valence state shows the distinctive absorption band characteristic of compound C, identified in the membrane-bound oxidase. The assignment of the alpha, beta, gamma and near-i.r. absorption bands to possible valence states of these compounds is made.
摘要
完全还原状态的可溶性细胞色素氧化酶在低温下与O₂的反应揭示了具有与膜结合氧化酶所观察到的特征相似的组分，即化合物A和B，分别被认为是“氧基”和“过氧基”化合物。在可溶性和膜结合氧化酶中都鉴定出了类似的物种；与膜结合物种相比，可溶性制剂中与O₂反应的反应速度常数和解离常数降低了2-3倍，这是由于前者对O₂的反应性降低所致。以混合价态制备的氧化酶显示出在膜结合氧化酶中鉴定出的化合物C的独特吸收带。对这些化合物可能的价态进行了α、β、γ和近红外吸收带的归属。
相似文献
1
Low-temperature kinetics of the reaction of oxygen and solubilized cytochrome oxidase.氧气与可溶性细胞色素氧化酶反应的低温动力学
Biochem J. 1978 Jun 1;171(3):787-98. doi: 10.1042/bj1710787.
2
Characterization of the intermediates in the reaction of membrane-bound mixed-valence-state cytochrome oxidase with oxygen at low temperatures by optical spectroscopy in the visible region.通过可见区域的光谱学对膜结合混合价态细胞色素氧化酶在低温下与氧气反应中的中间体进行表征。
Biochem J. 1980 Jun 1;187(3):617-22. doi: 10.1042/bj1870617.
3
CO binding to mitochondrial mixed valence state cytochrome oxidase at low temperatures.低温下一氧化碳与线粒体混合价态细胞色素氧化酶的结合
Biochim Biophys Acta. 1980 Mar 7;590(1):34-49. doi: 10.1016/0005-2728(80)90144-9.
4
Spectroscopic evidence for the participation of compound A (Fea32+-O2) in the reaction of mixed-valence cytochrome c oxidase with oxygen at room temperature.化合物A（Fea32+-O2）在室温下参与混合价态细胞色素c氧化酶与氧气反应的光谱学证据。
Biochem J. 1983 Dec 1;215(3):659-67. doi: 10.1042/bj2150659.
5
Oxygen intermediates and mixed valence states of cytochrome oxidase: infrared absorption difference spectra of compounds A, B, and C of cytochrome oxidase and oxygen.氧中间体与细胞色素氧化酶的混合价态：细胞色素氧化酶与氧的化合物A、B和C的红外吸收差光谱
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4777-80. doi: 10.1073/pnas.74.11.4777.
6
Functional intermediates in the reaction of membrane-bound cytochrome oxidase with oxygen.膜结合细胞色素氧化酶与氧气反应中的功能中间体。
J Biol Chem. 1975 Dec 25;250(24):9226-37.
7
Oxygen kinetics of frozen cytochrome oxidase. The capacity of the oxygen pocket.冷冻细胞色素氧化酶的氧动力学。氧腔的容量。
Biochem J. 1980 Feb 1;185(2):527-30. doi: 10.1042/bj1850527.
8
Reaction of caa3-type terminal cytochrome oxidase from the thermophilic bacterium PS3 with oxygen and carbon monoxide at low temperatures.嗜热细菌PS3的caa3型末端细胞色素氧化酶在低温下与氧气和一氧化碳的反应。
Biochem J. 1984 Jul 15;221(2):529-33. doi: 10.1042/bj2210529.
9
Resolution of two compound C-type intermediates in the reaction with oxygen of mixed-valence state membrane-bound cytochrome oxidase.混合价态膜结合细胞色素氧化酶与氧反应中两种复合C型中间体的解析。
Biochim Biophys Acta. 1981 Jan 14;634(1):30-40. doi: 10.1016/0005-2728(81)90125-0.
10
Kinetic and ligand binding evidence for two heme A-based terminal oxidases in plasma membranes from Bacillus subtilis.枯草芽孢杆菌质膜中两种基于血红素A的末端氧化酶的动力学和配体结合证据。
Arch Biochem Biophys. 1993 Feb 15;301(1):129-37. doi: 10.1006/abbi.1993.1124.
引用本文的文献
1
Reaction of Mixed Valence State Cytochrome Oxidase with Oxygen in Plant Mitochondria: A STUDY BY LOW TEMPERATURE FLASH PHOTOLYSIS AND RAPID WAVELENGTH SCANNING OPTICAL SPECTROMETRY.植物线粒体中混合价态细胞色素氧化酶与氧气的反应：低温闪光光解和快速波长扫描光谱法研究
Plant Physiol. 1981 Jul;68(1):229-35. doi: 10.1104/pp.68.1.229.
2
Carbon monoxide- and oxygen-reacting haemoproteins in the mitochondrial fraction from the soil amoeba Acanthamoeba castellanii. Studies at subzero temperatures.土壤变形虫卡氏棘阿米巴线粒体组分中一氧化碳和氧气反应性血红蛋白。零下温度下的研究。
Biochem J. 1981 Nov 15;200(2):337-42. doi: 10.1042/bj2000337.
3
Characterization of the intermediates in the reaction of membrane-bound mixed-valence-state cytochrome oxidase with oxygen at low temperatures by optical spectroscopy in the visible region.通过可见区域的光谱学对膜结合混合价态细胞色素氧化酶在低温下与氧气反应中的中间体进行表征。
Biochem J. 1980 Jun 1;187(3):617-22. doi: 10.1042/bj1870617.
4
The reaction of fully reduced cytochrome c oxidase with oxygen studied by flow-flash spectrophotometry at room temperature. Evidence for new pathways of electron transfer.在室温下通过流动闪光分光光度法研究完全还原的细胞色素c氧化酶与氧气的反应。电子转移新途径的证据。
Biochem J. 1984 Mar 15;218(3):913-21. doi: 10.1042/bj2180913.
5
Structural features and the reaction mechanism of cytochrome oxidase: iron and copper X-ray absorption fine structure.细胞色素氧化酶的结构特征与反应机制：铁和铜的X射线吸收精细结构
Biophys J. 1981 Jun;34(3):465-98. doi: 10.1016/S0006-3495(81)84863-1.
6
Oxygen kinetics of frozen cytochrome oxidase. The capacity of the oxygen pocket.冷冻细胞色素氧化酶的氧动力学。氧腔的容量。
Biochem J. 1980 Feb 1;185(2):527-30. doi: 10.1042/bj1850527.
7
Characterization of the low-temperature intermediates of the reaction of fully reduced soluble cytochrome oxidase with oxygen by electron-paramagnetic-resonance and optical spectroscopy.通过电子顺磁共振和光谱学对完全还原的可溶性细胞色素氧化酶与氧气反应的低温中间体进行表征。
Biochem J. 1980 Jan 1;185(1):139-54. doi: 10.1042/bj1850139.
8
Reaction of caa3-type terminal cytochrome oxidase from the thermophilic bacterium PS3 with oxygen and carbon monoxide at low temperatures.嗜热细菌PS3的caa3型末端细胞色素氧化酶在低温下与氧气和一氧化碳的反应。
Biochem J. 1984 Jul 15;221(2):529-33. doi: 10.1042/bj2210529.
9
Redox analysis of the cytochrome o-type quinol oxidase complex of Escherichia coli reveals three redox components.大肠杆菌细胞色素o型醌氧化酶复合物的氧化还原分析揭示了三种氧化还原成分。
Biochem J. 1991 Mar 15;274 ( Pt 3)(Pt 3):723-30. doi: 10.1042/bj2740723.
10
The reaction of cytochrome omicron in Escherichia coli with oxygen. Low-temperature kinetic and spectral studies.大肠杆菌中细胞色素奥米克戎与氧气的反应。低温动力学和光谱研究。
Biochem J. 1979 Nov 15;184(2):379-89. doi: 10.1042/bj1840379.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
KINETIC OBSERVATIONS ON THE NEAR INFRARED BAND OF CYTOCHROME C OXIDASE.细胞色素C氧化酶近红外波段的动力学观测
J Biol Chem. 1965 Jun;240:2694-8.
3
Studies on cytochrome a. IX. Significance of oxygenated cytochrome a in the cytochrome oxidase mechanism.细胞色素a的研究。IX. 氧化型细胞色素a在细胞色素氧化酶机制中的意义。
J Biochem. 1963 Jun;53:489-99. doi: 10.1093/oxfordjournals.jbchem.a127728.
4
Studies of the electron transport system. XXXVI. Properties of copper in cytochroma oxidase.电子传递系统的研究。三十六。细胞色素氧化酶中铜的性质。
J Biol Chem. 1961 Jun;236:1857-62.
5
Photolysis kinetics by frequency doubled laser emission.倍频激光发射的光解动力学
FEBS Lett. 1969 Jun;3(4):287-291. doi: 10.1016/0014-5793(69)80160-2.
6
New species of the "oxygenated compound" of cytochrome oxidase.细胞色素氧化酶“氧化合物”的新物种。
FEBS Lett. 1972 Mar 15;21(2):199-202. doi: 10.1016/0014-5793(72)80136-4.
7
Reaction of cytochrome C oxidase with CO: involvement of the invisible copper.细胞色素C氧化酶与一氧化碳的反应：不可见铜的作用
FEBS Lett. 1974 Nov 1;48(1):45-9. doi: 10.1016/0014-5793(74)81058-6.
8
Studies on partially reduced mammalian cytochrome oxidase. Reactions with carbon monoxide and oxygen.部分还原的哺乳动物细胞色素氧化酶的研究。与一氧化碳和氧气的反应。
Biochem J. 1974 Feb;137(2):205-15. doi: 10.1042/bj1370205.
9
Studies on cytochrome oxidase. 8. Preparation and some properties of cardiac cytochrome oxidase.细胞色素氧化酶的研究。8. 心肌细胞色素氧化酶的制备及其某些特性
J Biol Chem. 1972 Oct 25;247(20):6375-83.
10
Studies on the electron transport chain at subzero temperatures: electron transport at site 3.
Arch Biochem Biophys. 1972 Jul;151(1):304-15. doi: 10.1016/0003-9861(72)90501-2.
Zotero 插件