Animal Health Service (GD-Deventer), 7400 AA, Deventer, The Netherlands.
Vet Rec. 2010 Sep 18;167(12):451-4. doi: 10.1136/vr.c4274.
A herd of pigs being reared for breeding and fattening, in which there had been incidences of abortion and wasting, reduced growth rates and an increase in mortality for the past year, were tested for Mycobacterium infection by pathological examinations, skin test, serology and Mycobacterium culture. In one placenta, and also in the lung tissues of fetuses, Ziehl-Neelsen staining revealed acid-fast bacilli in combination with infiltrations of neutrophils, macrophages and multinucleated giant cells. Acid-fast bacilli were also found in the mesenteric lymph nodes, liver and/or spleen and jejunum of pigs with wasting and in slaughtered animals. The specimen cultures were identified as Mycobacterium avium subspecies hominissuis using IS1245-specific PCR and IS1245 restriction fragment length polymorphism (RFLP). IS1245 RFLP revealed that the herd was infected with multiple M avium subspecies hominissuis strains belonging to at least two different clades. It is suggested that this infection may have played a more important role in the economic losses of the pig farm than had been assumed previously.
一群用于繁殖和育肥的猪，在过去的一年中发生了流产和消瘦、生长速度降低以及死亡率增加的情况，通过病理检查、皮肤试验、血清学和分枝杆菌培养对其进行了分枝杆菌感染测试。在一个胎盘中，以及胎儿的肺组织中，齐尔-尼尔森染色显示抗酸杆菌与中性粒细胞、巨噬细胞和多核巨细胞的浸润相结合。在消瘦的猪和屠宰动物的肠系膜淋巴结、肝脏和/或脾脏和空肠中也发现了抗酸杆菌。使用 IS1245 特异性 PCR 和 IS1245 限制性片段长度多态性（RFLP）对标本培养物进行鉴定，结果表明这些猪感染了属于至少两个不同分支的多种禽分枝杆菌亚种。这表明这种感染可能在养猪场的经济损失中比以前认为的更为重要。
