Perl-Treves R, Abu-Abied M, Magal N, Galun E, Zamir D
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Biochem Genet. 1990 Oct;28(9-10):543-52. doi: 10.1007/BF00554381.
The isozyme pattern of superoxide dismutase (SOD) in tomato consists of two Cu,Zn isozymes located, respectively, in the chloroplast and in the cytosol, as well as additional isozymes of the Mn or Fe SOD type. We have shown that SOD-1 is the chloroplastic Cu,Zn SOD and is related to cDNA clone T10. Restriction fragment length polymorphism (RFLP) analysis was performed with two cDNA clones representing tomato Cu,Zn-superoxide dismutases. T10, coding for the chloroplast isozyme, was thus mapped to chromosome 11, between marker TG46 and TG108, while clone P31, coding for the cytosolic Cu,Zn SOD isozyme, was mapped to chromosome 1 between TG24 and TG81. SOD is associated with the response of plants to various environmental stresses; the mapping information presented here would permit the demonstration of this association by genetic analysis.
番茄中超氧化物歧化酶（SOD）的同工酶模式由分别位于叶绿体和细胞质中的两种铜锌同工酶以及锰或铁SOD类型的其他同工酶组成。我们已经表明，SOD-1是叶绿体铜锌SOD，与cDNA克隆T10相关。用两个代表番茄铜锌超氧化物歧化酶的cDNA克隆进行了限制性片段长度多态性（RFLP）分析。编码叶绿体同工酶的T10因此被定位到11号染色体上，在标记TG46和TG108之间，而编码细胞质铜锌SOD同工酶的克隆P31被定位到1号染色体上，在TG24和TG81之间。SOD与植物对各种环境胁迫的反应有关；此处提供的定位信息将允许通过遗传分析证明这种关联。
