Department of Special Pathogens, International Research Center for Infectious Diseases, University of Tokyo, Tokyo, Japan.
Rev Med Virol. 2010 Nov;20(6):380-91. doi: 10.1002/rmv.666.
The influenza A virus genome consists of eight segmented, single-stranded, negative-sense RNAs. Each viral RNA (vRNA) segment forms a ribonucleoprotein (RNP) complex together with NPs and a polymerase complex, which is a fundamental unit for transcription and replication of the viral genome. Although the exact structure of the intact RNP remains poorly understood, recent electron microscopic studies have revealed certain structural characteristics of the RNP. This review focuses on the findings of these various electron microscopic analyses of RNPs extracted from virions and RNPs inside virions. Based on the morphological and structural observations, we present the architecture of RNPs within a virion and discuss the genome packaging mechanism by which the vRNA segments are incorporated into virions.
甲型流感病毒基因组由八个分段的、单链的、负义 RNA 组成。每个病毒 RNA（vRNA）片段与 NPs 和聚合酶复合物一起形成核糖核蛋白（RNP）复合物，这是病毒基因组转录和复制的基本单位。尽管完整 RNP 的确切结构仍不清楚，但最近的电子显微镜研究揭示了 RNP 的某些结构特征。本综述重点介绍了从病毒粒子中提取的 RNP 和病毒粒子内的 RNP 的各种电子显微镜分析的结果。基于形态和结构观察，我们展示了病毒粒子内 RNP 的结构，并讨论了 vRNA 片段被包装到病毒粒子中的基因组包装机制。
