Guangdong Higher Education Key Laboratory of Ecology and Environmental Science, South China Normal University, Guangzhou 510631, China.
Environ Toxicol Chem. 2010 Nov;29(11):2607-15. doi: 10.1002/etc.321.
The current study investigated the development and reproductive health of western mosquitofish (Gambusia affinis) in the Hanxi River, which has been heavily contaminated by municipal wastewaters from towns in Dongguan of southern China. Western mosquitofish collected from four study sites, Songmu (SM), Yangwu (YW), Hengli (HL), and Zhangcun (ZC) of the Hanxi River, as well as a reference site (LX) of the Liuxi River, were dissected and analyzed for development parameters (total length, wet body mass, liver mass, gonad index, and population composition), reproductive parameters (oocyte count and weight of females, and sperm count and viability of males), and morphology (anal fin in males and females, and hemal spines in males). With the exception of the origin site (SM), mosquitofish from the Hanxi River exhibited significantly decreasing development and reproduction levels. Significant correlations were found for the collected mosquitofish between the morphological and reproductive indexes (ratio of perpendicular distance to tip to vertical distance from the tip on the 16th hemal spine) versus the sperm count of males; ratio 16P:16D versus sperm viability of males; segments of anal fin ray 3 versus oocyte count of females; and segments of anal fin ray 3 versus average oocyte mass of females. The results demonstrated that both male and female mosquitofish in the Hanxi River were affected by the discharged wastewaters, as reflected in their morphological changes in comparison with those of mosquitofish from the reference site.
本研究调查了受中国南方东莞市城镇污水严重污染的寒溪河水系西部食蚊鱼（ Gambusia affinis ）的发育和生殖健康。从寒溪河流域的四个研究点（Songmu [SM]、Yangwu [YW]、Hengli [HL]和Zhangcun [ZC]）以及柳溪河流域的一个参考点（LX）采集了西部食蚊鱼，并对其进行了解剖和分析，以评估其发育参数（全长、湿体重、肝重、性腺指数和种群组成）、生殖参数（雌性的卵母细胞计数和体重，以及雄性的精子计数和活力）和形态（雄性和雌性的臀鳍、雄性的半棘）。除了起源点（SM）外，寒溪河中的食蚊鱼表现出明显的发育和生殖水平下降。对收集到的食蚊鱼进行了分析，发现其形态和生殖指标之间存在显著相关性（第 16 根半棘上从尖端到垂直距离的垂直距离与雄性精子计数之比；16P:16D 与雄性精子活力之比；3 号臀鳍鳍条与雌性卵母细胞计数之比；以及 3 号臀鳍鳍条与雌性平均卵母细胞质量之比）。结果表明，寒溪河中的雄性和雌性食蚊鱼都受到了排放废水的影响，这反映在它们的形态变化上，与参考点的食蚊鱼相比发生了变化。
