Ullrich Thomas, Wesenberg Dirk, Bleuel Corinna, Krauss Gerd-Joachim, Schmid Martin G, Weiss Michael, Gübitz Gerald
Faculty of Biosciences, Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3, D-06120 Halle (Saale), Germany.
Biomed Chromatogr. 2010 Oct;24(10):1125-9. doi: 10.1002/bmc.1415.
The development of methods for the separation of the enantiomers of fenoterol by chiral HPLC and capillary zone electrophoresis (CZE) is described. For the HPLC separation precolumn fluorescence derivatization with naphthyl isocyanate was applied. The resulting urea derivatives were resolved on a cellulose tris(3,5-dimethylphenylcarbamate)-coated silica gel column employing a column switching procedure. Detection was carried out fluorimetrically with a detection limit in the low ng/mL range. The method was adapted to the determination of fenoterol enantiomers in rat heart perfusates using liquid-liquid extraction. As an alternative a CE method was used for the direct separation of fenoterol enantiomers comparing different cyclodextrin derivatives as chiral selectors.
描述了通过手性高效液相色谱法（HPLC）和毛细管区带电泳法（CZE）分离非诺特罗对映体的方法。对于HPLC分离，采用了异氰酸萘酯进行柱前荧光衍生化。所得的脲衍生物在涂有三（3,5-二甲基苯基氨基甲酸酯）纤维素的硅胶柱上通过柱切换程序进行分离。采用荧光检测，检测限在低纳克/毫升范围内。该方法通过液-液萃取适用于测定大鼠心脏灌注液中的非诺特罗对映体。作为替代方法，使用CE方法直接分离非诺特罗对映体，比较了不同的环糊精衍生物作为手性选择剂。
