Marangoni S, Ghiso J, Sampaio S V, Arantes E C, Giglio J R, Oliveira B, Frangione B
Department of Pathology, New York University Medical Center, New York 10016.
J Protein Chem. 1990 Oct;9(5):595-601. doi: 10.1007/BF01025013.
The complete sequence of the toxin TsTX-VI from the venom of the scorpion Tityus serrulatus Lutz and Mello is presented. The sequence has been determined by automated Edman analysis of the reduced and carboxymethylated protein as well as of the resulting peptides, obtained from S. aureus protease and tryptic digestions. TsTX-VI is composed of 62 residues and has a calculated molecular weight of 6717. Homology studies with other scorpion toxins show that TsTX-VI is more similar to the Old World than to the North American scorpion toxins. The hydropathic index indicates that TsTX-VI is more hydrophobic than Ts-gamma. Toxicity studies carried out in mice demonstrate that i.v. injection of TsTX-VI is unable to evoke the usual symptoms induced by the typical neurotoxins of this venom, but only a generalized allergic reaction. These properties are important in clarifying the relationship between primary structure and biological function of scorpion toxins.
本文给出了来自锯齿钝尾蝎毒液的毒素TsTX-VI的完整序列。该序列通过对还原和羧甲基化的蛋白质以及从金黄色葡萄球菌蛋白酶和胰蛋白酶消化产物中获得的肽段进行自动埃德曼分析来确定。TsTX-VI由62个残基组成，计算分子量为6717。与其他蝎毒素的同源性研究表明，TsTX-VI与旧大陆蝎毒素的相似性高于北美蝎毒素。亲水性指数表明，TsTX-VI比Ts-γ更疏水。在小鼠身上进行的毒性研究表明，静脉注射TsTX-VI不会引发该毒液典型神经毒素所引起的常见症状，而只会引发全身性过敏反应。这些特性对于阐明蝎毒素的一级结构与生物学功能之间的关系很重要。
