[急性胰腺炎后急性肺损伤中分泌型Ⅱ型磷脂酶A₂的表达及清胰汤对其的干预作用]

[Expression of secretory type II phospholipase A₂ in acute lung injury following acute pancreatitis and interventional effect of Qingyi decoction on it].

作者信息

Zhang Xue-mei, Chen Hai-long, Wang Zhao-hui

机构信息

Department of General Surgery, the PLA 210th Hospital, Dalian 116023, Liaoning, China.

出版信息

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Sep;22(9):518-21.

PMID:20854728

Abstract

OBJECTIVE

To investigate the expression of secretory type II phospholipase A(2) (sPLA(2)-II) in lung of rats with acute lung injury (ALI) complicating severe acute pancreatitis (SAP), and the effect of Qingyi decoction (QYT) on ALI.

METHODS

Thirty Sprague Dawley (SD) rats were randomly divided into three groups: sham operation (SO) group, model group and QYT group, with 10 rats in each group. SAP model was reproduced by reverse injection of sodium deoxycholate into the common bile- pancreatic duct of rats. The pancreas of rats was just exposed in SO group. QYT (10 ml/kg) was gavaged 30 minutes and 12 hours after SAP was induced in QYT group. The blood gas analysis was performed 24 hours after operation. Serum amylase (AMY) levels, sPLA(2) and lung wet/dry ratio (W/D) were determined. The sPLA(2)-II mRNA and sPLA(2)-II protein expression in lung were detected by reverse transcription- polymerase chain reaction (RT-PCR) and Western blotting. The pathological changes in lung and pancreas were observed.

RESULTS

Compared with SO group, the levels of arterial partial pressure of oxygen (PaO(2)) and pH value in model group were significantly decreased [PaO(2) (mm Hg, 1 mm Hg=0.133 kPa): 79.24±5.84 vs. 96.78±3.81, pH value: 7.269±0.054 vs. 7.391±0.054], arterial partial pressure of carbon dioxide (PaCO(2)), the serum levels of AMY, W/D ratio and the serum levels of sPLA(2) were significantly increased [PaCO(2) (mm Hg): 47.57±2.55 vs. 27.69±1.02, AMY (U/L): 7 144.19±727.91 vs. 1 193.41±192.54, W/D ratio: 8.57±2.45 vs. 3.70±0.90, sPLA(2) (nmol×min(-1) ×ml(-1)): 45.13±6.05 vs. 29.94±6.39], the expression of sPLA(2)-II mRNA (1.28±0.21 vs. 0.80±0.08) and protein were significantly increased (all P <0.05). Compared with model group, blood PaO(2) and pH value were significantly increased [PaO(2): (88.16±5.07) mm Hg, pH value: 7.322±0.039], the PaCO(2), the serum levels of AMY, W/D ratio and the serum levels of sPLA(2) in QYT group were significantly decreased [PaCO(2): (33.13±2.14) mm Hg, AMY: (4 283.51±527.52) U/L, W/D ratio: 4.05±0.52, sPLA(2): (28.00±4.78) nmol×min(-1) ×ml(-1)], and the expression of sPLA(2)-II mRNA (0.89±0.08) and protein were significantly decreased (all P <0.05). The pathological changes in lung and pancreas in QYT group were milder than those in SAP group.

CONCLUSION

The higher expression of sPLA(2)-IIin lung may be one of pathogenetic factors in ALI induced by SAP. Administration of QYT can reduce the injury of lung by decreasing the expression of sPLA(2)-II in transcriptional level and thus protecting pulmonary function.

摘要

目的

探讨分泌型Ⅱ型磷脂酶A2（sPLA2-Ⅱ）在重症急性胰腺炎（SAP）并发急性肺损伤（ALI）大鼠肺组织中的表达，以及清胰汤（QYT）对ALI的影响。

方法

将30只Sprague Dawley（SD）大鼠随机分为三组：假手术（SO）组、模型组和QYT组，每组10只。通过逆行向大鼠胆总管内注射脱氧胆酸钠复制SAP模型。SO组仅暴露大鼠胰腺。QYT组在诱导SAP后30分钟和12小时给予QYT（10 ml/kg）灌胃。术后24小时进行血气分析。测定血清淀粉酶（AMY）水平、sPLA2及肺组织湿/干比（W/D）。采用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法检测肺组织中sPLA2-Ⅱ mRNA和sPLA2-Ⅱ蛋白表达。观察肺和胰腺的病理变化。

结果

与SO组比较，模型组动脉血氧分压（PaO2）和pH值显著降低[PaO2（mmHg，1 mmHg = 0.133 kPa）：79.24±5.84比96.78±3.81，pH值：7.269±0.054比7.391±0.054]，动脉血二氧化碳分压（PaCO2）、血清AMY水平、W/D比值及血清sPLA2水平显著升高[PaCO2（mmHg）：47.57±2.55比27.69±1.02，AMY（U/L）：7 144.19±727.91比1 193.41±192.54，W/D比值：8.57±2.45比3.70±0.90，sPLA2（nmol×min-1×ml-1）：45.13±6.05比29.94±6.39]，sPLA2-Ⅱ mRNA（1.28±0.21比0.80±0.08）和蛋白表达显著增加（均P <0.05）。与模型组比较，QYT组血PaO2和pH值显著升高[PaO2：（88.16±5.07）mmHg，pH值：7.322±0.039]，PaCO2、血清AMY水平、W/D比值及血清sPLA2水平显著降低[PaCO2：（33.13±2.14）mmHg，AMY：（4 283.51±527.52）U/L，W/D比值：4.05±0.52，sPLA2：（28.00±4.78）nmol×min-1×ml-1]，sPLA2-Ⅱ mRNA（0.89±0.08）和蛋白表达显著降低（均P <0.05）。QYT组肺和胰腺的病理变化较SAP组轻。