通过低温还原/退火、EPR/ENDOR 研究揭示血红素单加氧酶反应中的活性中间体。
Active intermediates in heme monooxygenase reactions as revealed by cryoreduction/annealing, EPR/ENDOR studies.
机构信息
Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
出版信息
Arch Biochem Biophys. 2011 Mar 1;507(1):36-43. doi: 10.1016/j.abb.2010.09.013. Epub 2010 Sep 18.
Abstract
This review describes the use of cryoreduction/annealing EPR/ENDOR techniques for determining the active oxidizing species in reactions catalyzed by heme monooxygenases. The three candidate heme states are: ferric peroxo, ferric hydroperoxo and compound I intermediates. The enzymes discussed include cytochromes P450, nitric oxide synthase and heme oxygenase.
摘要
这篇综述描述了使用低温还原/退火电子顺磁共振/电子-核双共振（EPR/ENDOR）技术来确定血红素单加氧酶催化反应中的活性氧化物种。这三种候选的血红素状态是：铁过氧、铁过氢氧和化合物 I 中间体。讨论的酶包括细胞色素 P450、一氧化氮合酶和血红素加氧酶。
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