Biorefinery Research Institute, Department of Paper and Bioprocess Engineering, College of Environment Science and Forestry, State University of Newyork, 1 Forestry Road, Syracuse, NY 13210, USA.
Bioresour Technol. 2011 Jan;102(2):2133-6. doi: 10.1016/j.biortech.2010.08.097. Epub 2010 Aug 31.
In this study the influence of the insoluble solids in nano-filtrated sugar maple hemicellulosic hydrolysate on the metabolic behavior of Pichia stiptis was investigated. The particle properties of hemicellulosic hydrolysate were analyzed. Phosphoric acid and ammonium (PA) were applied to remove the particles. The metabolic behavior and growth property of P. stipitis in particle--removed hydrolysate was measured. Results demonstrated that the average particle size and zeta potential of the untreated hydrolysate were 2266.9±78.2 nm and -6.09±0.49 mV. Xylose consumption and ethanol production rate were significantly decreased when particle content is greater than 1.63 g/L. Because the majority of particles (34 g/L) were removed from hydrolysates by phosphoric acid and ammonium treatment, the fermentability of the hydrolysate was significantly improved. These results indicated particles play an important role in hydrolysate inhibition effect.
在这项研究中，考察了纳米过滤糖枫半纤维素水解液中不溶性固体对毕赤酵母代谢行为的影响。分析了半纤维素水解液的颗粒性质。采用磷酸和氨（PA）去除颗粒。测定了去除颗粒后的水解液中毕赤酵母的代谢行为和生长特性。结果表明，未经处理的水解液的平均粒径和 Zeta 电位分别为 2266.9±78.2nm 和-6.09±0.49mV。当颗粒含量大于 1.63g/L 时，木糖消耗和乙醇生成速率显著降低。由于磷酸和氨处理从水解液中去除了大部分颗粒（34g/L），因此水解液的发酵性能得到了显著提高。这些结果表明颗粒在水解液抑制作用中起着重要作用。
