Unité de Génétique Moléculaire Animale, Université de Limoges Faculté des Sciences et Techniques, INRA UMR 1061, 123 Avenue Albert Thomas, 87060 Limoges, France.
J Biol Chem. 2010 Dec 3;285(49):38399-414. doi: 10.1074/jbc.M110.163931. Epub 2010 Sep 20.
Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. β-Galactoside α2,6-sialyltransferases ST6Gal I and ST6Gal II are the two unique members of the ST6Gal family described in higher vertebrates. The availability of genome sequences enabled the identification of more distantly related invertebrates' st6gal gene sequences and allowed us to propose a scenario of their evolution. Using a phylogenomic approach, we present further evidence of an accelerated evolution of the st6gal1 genes both in their genomic regulatory sequences and in their coding sequence in reptiles, birds, and mammals known as amniotes, whereas st6gal2 genes conserve an ancestral profile of expression throughout vertebrate evolution.
唾液酸转移酶是唾液酸糖缀合物生物合成中的关键酶，可催化唾液酸残基从其活化形式转移到寡糖受体上。β-半乳糖苷α2,6-唾液酸转移酶 ST6Gal I 和 ST6Gal II 是高等脊椎动物中 ST6Gal 家族的两个独特成员。基因组序列的出现使得能够鉴定出更远缘的无脊椎动物 st6gal 基因序列，并使我们能够提出它们的进化情景。使用系统基因组学方法，我们进一步证明了 st6gal1 基因在爬行动物、鸟类和哺乳动物（称为羊膜动物）中的基因组调控序列和编码序列中经历了加速进化，而 st6gal2 基因在整个脊椎动物进化过程中保持着祖先的表达模式。
