Instituto de Investigaciones Bioquímicas de La Plata, CONICET, Facultad de Ciencias Médicas, UNLP, calles 60 y 120 (1900) La Plata, Argentina.
J Econ Entomol. 2010 Aug;103(4):1094-9. doi: 10.1603/ec10072.
We studied the effect of the entomopathogenic fungus Beauveria bassiana strain GHA on a) colony development of the beetles Tribolium castaneum (Herbst) and Ulomoides dermestoides (Fairmaire) (Coleoptera: Tenebrionidae) under laboratory conditions; and 2) the volatile blend released by both beetles, containing defensive pheromones, by using the solid phase microextraction technique. Colony development of both species was strongly altered 3 mo after treatment with B. bassiana, showing a significant reduction in progeny of 37.5% for T. castaneum and 50.0% for U. dermestoides. We also showed that the volatiles released by T. castaneum diminished close to 20% compared with those of healthy beetles, whereas in U. dermestoides secretions dramatically dropped to 5%, 7 d after immersion in 1 x 10(9) conidia per ml. These results suggest that after infection events take place, fungus-induced diminished secretion of the defensive pheromones may be a physiologic clue for behavioral changes in infected beetles.
我们研究了昆虫病原真菌球孢白僵菌菌株 GHA 对以下两方面的影响：a）在实验室条件下，对甲虫赤拟谷盗（Herbst）和皮蠹（Coleoptera：Tenebrionidae）的种群发展的影响；2）使用固相微萃取技术，对两种甲虫释放的挥发性混合物（含有防御信息素）的影响。感染球孢白僵菌 3 个月后，两种甲虫的种群发展均受到强烈干扰，赤拟谷盗的后代数量显著减少 37.5%，皮蠹的后代数量减少 50.0%。我们还表明，与健康甲虫相比，赤拟谷盗释放的挥发性物质减少了近 20%，而皮蠹在浸入 1 x 10(9)个分生孢子/ml 后 7 天，分泌物急剧减少到 5%。这些结果表明，在感染事件发生后，真菌诱导的防御信息素分泌减少可能是感染甲虫行为变化的生理线索。
