Oka M T, Arai T, Hamaguchi Y
Biological Laboratory, Faculty of Science, Tokyo Institute of Technology, Japan.
Cell Struct Funct. 1990 Dec;15(6):373-8. doi: 10.1247/csf.15.373.
Two monoclonal antibodies against alpha-tubulin (YL1/2 and D2D6) were microinjected into the egg of the sand dollar Clypeaster japonicus, and their effects on cleavage of the egg were investigated. They had already been shown by immunoblotting to react specifically with egg tubulin and by immunofluorescence to stain the mitotic apparatus [OKA et al., (1990). Cell Motil. Cytoskel. 16:239-250]. Injection of YL1/2 prevented chromosome movement and cleavage, although the cleavage furrow developed in some cases. In all eggs injected at prometaphase, metaphase, or anaphase, the birefringence of the mitotic apparatus disappeared immediately after injection. Injection of D2D6 had no significant effect on mitosis or cleavage of whole eggs injected after nuclear disappearance, although it prevented the disappearance of the nuclear envelope in 54% of the eggs injected before the disappearance. FITC-conjugated D2D6 did not accumulate in the spindle when injected into the dividing sand dollar egg. These results indicate that YL1/2 disassembled microtubules, whereas D2D6 did not bind to microtubules in the living cell.
将两种抗α-微管蛋白的单克隆抗体（YL1/2和D2D6）显微注射到日本饼海胆的卵中，研究它们对卵裂的影响。免疫印迹已表明它们能与卵微管蛋白特异性反应，免疫荧光显示它们能对有丝分裂器进行染色[冈田等人，（1990年）。细胞运动与细胞骨架。16:239 - 250]。注射YL1/2可阻止染色体移动和卵裂，不过在某些情况下会形成分裂沟。在所有于前中期、中期或后期注射的卵中，有丝分裂器的双折射在注射后立即消失。注射D2D6对核消失后注射的完整卵的有丝分裂或卵裂没有显著影响，尽管它能阻止54%在核消失前注射的卵中核膜的消失。当将异硫氰酸荧光素（FITC）偶联的D2D6注射到正在分裂的饼海胆卵中时，它不会在纺锤体中积累。这些结果表明，YL1/2会拆解微管，而D2D6在活细胞中不与微管结合。
