Modern Biological Research Center, Yunnan University, Kunming 650091, China.
J Theor Biol. 2010 Dec 21;267(4):495-501. doi: 10.1016/j.jtbi.2010.09.001. Epub 2010 Sep 19.
We have published the dynamic extended folding (DEF) method, which is a RNA secondary structure prediction approach-to simulate the in vivo RNA co-transcriptional folding process. In order to verify the reliability of the method, we selected the X-ray-determined Tetrahymena group I intron as a sample to construct the framework of its folding secondary structure. Our prediction coincides well with the secondary structure predicted by T.R. Cech and the X-ray diffraction crystal structure determined by Lehnert V. Our results show that the DEF framework structure of Tetrahymena group I intron reflects its function sites in a concise and straightforward manner, and the scope of the simulation was expanded.
我们已经发表了动态扩展折叠（DEF）方法，这是一种 RNA 二级结构预测方法——模拟体内 RNA 共转录折叠过程。为了验证该方法的可靠性，我们选择了 X 射线测定的四膜虫组 I 内含子作为样本，构建其折叠二级结构的框架。我们的预测与 T.R.切赫预测的二级结构和莱纳特 V 确定的 X 射线衍射晶体结构非常吻合。我们的结果表明，四膜虫组 I 内含子的 DEF 框架结构简明扼要地反映了其功能部位，并且扩大了模拟的范围。
