鹌鹑（日本鹌鹑）输卵管细胞原代培养物中胰岛素受体的特性。胰岛素受体水平受类固醇和肽类激素调节。
Characterization of insulin receptors in primary cultures of quail (Coturnix coturnix japonica) oviduct cells. The level of insulin receptor is regulated by steroid and peptide hormones.
作者信息
Kato S, Itoh S, Miura Y, Naito H, Noguchi T
机构信息
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
出版信息
Comp Biochem Physiol B. 1990;97(4):783-91. doi: 10.1016/0305-0491(90)90123-b.
PMID:2085960
Abstract
- We have characterized the insulin receptor in primary cultured quail oviduct cells and examined the hormonal regulation of its level. 2. We have also shown the recycling pathway of insulin receptors in the cultured cells using specific inhibitors (tunicamycin, chloroquine, monensin, and brefeldin A). 3. Our data suggest that glucocorticoids play important physiological roles in egg-white protein synthesis through increasing the number of insulin receptors and insulin through enhancing the transport of amino acids.
摘要
- 我们已对原代培养的鹌鹑输卵管细胞中的胰岛素受体进行了表征，并研究了其水平的激素调节。2. 我们还利用特异性抑制剂（衣霉素、氯喹、莫能菌素和布雷菲德菌素A）在培养细胞中展示了胰岛素受体的循环途径。3. 我们的数据表明，糖皮质激素通过增加胰岛素受体的数量在蛋清蛋白合成中发挥重要生理作用，而胰岛素则通过增强氨基酸转运发挥作用。
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