Plant Research International, Droevendaalsesteeg, The Netherlands.
Plant Signal Behav. 2010 Oct;5(10):1260-2. doi: 10.4161/psb.5.10.12949. Epub 2010 Oct 1.
To study the importance of intercellular transport for MADS domain transcription factor functioning during floral development, we analyzed the dynamic behavior of fluorescently-tagged MADS domain proteins in transgenic plants by Confocal Laser Scanning Microscopy. These analyses, described in a recent paper in The Plant Journal, provided proof for previous suggestions that the Arabidopsis thaliana C-type protein AGAMOUS has a non-cell-autonomous role in floral meristem integrity. Furthermore, it indicated a possible non-cell-autonomous role for the B-type proteins APETALA3 and PISTILLATA, and the E-type protein SEPALLATA3, through lateral intercellular movement in the floral meristem. In this addendum we compare some of the available fluorescent protein-based technologies for the investigation of transcription factor movements and dynamics.
为了研究细胞间运输在花发育过程中 MADS 结构域转录因子功能中的重要性,我们通过共焦激光扫描显微镜分析了转荧光标记 MADS 结构域蛋白的转基因植物中的动态行为。这些分析结果在最近发表在《植物杂志》上的一篇论文中得到了证实,该论文表明拟南芥 C 型蛋白 AGAMOUS 在花分生组织完整性中具有非细胞自主的作用。此外,它还表明 B 型蛋白 APETALA3 和 PISTILLATA 以及 E 型蛋白 SEPALLATA3 可能通过花分生组织中的侧向细胞间运动发挥非细胞自主的作用。在本附录中,我们比较了一些现有的基于荧光蛋白的技术,用于研究转录因子的运动和动力学。