Hoekstra A G, Doornbos R M, Deurloo K E, Noordmans H J, Grooth B G, Sloot P M
Appl Opt. 1994 Jan 20;33(3):494-500. doi: 10.1364/AO.33.000494.
The complete scattering matrix S of spheres was measured with a flow cytometer. The experimental equipment allows simultaneous detection of two scattering-matrix elements for every sphere in the distribution. Two-parameter scatterplots with x and y coordinates determined by the S(ll) + S(ij) and S(ll)-S(ij) values are measured. Samples of spheres with very narrow size distributions (< 1%) were analyzed with a FlowCytometer, and they produced unexpected two-parameter scatterplots. Instead of compact distributions we observed Lissajous-like loops. Simulation of the scatterplots, using Lorenz-Mie theory, shows that these loops are due not to experimental errors but to true Lorenz-Mie scattering. It is shown that the loops originate from the sensitivity of the scattered field on the radius of the spheres. This paper demonstrates that the interpretation of rare events and hidden features in flow cytometry needs reconsideration.
用流式细胞仪测量了球体的完整散射矩阵S。该实验设备能够同时检测分布中每个球体的两个散射矩阵元素。测量了由S(ll)+S(ij)和S(ll)-S(ij)值确定x和y坐标的双参数散点图。用流式细胞仪分析了尺寸分布非常窄(<1%)的球体样本，得到了意想不到的双参数散点图。我们观察到的不是紧凑分布，而是类似李萨如图形的环路。使用洛伦兹-米氏理论对散点图进行模拟表明，这些环路不是由实验误差引起的，而是由真实的洛伦兹-米氏散射引起的。结果表明，这些环路源于散射场对球体半径的敏感性。本文表明，流式细胞术中罕见事件和隐藏特征的解释需要重新考虑。
