Wang J, Jiang Y, He A
Pathology Department of No.202 Hospital of PLA, Shenyang 110003, P.R.China.
Zhongguo Fei Ai Za Zhi. 1998 Jul 10;1(1):40-1. doi: 10.3779/j.issn.1009-3419.1998.01.11.
To explore the usefulness of p53 immunohistochemical staining as a marker of malignancy in the diagnosis of lung cancer.
The expression of p53 in 57 samples of pleural effusion and 69 bronchial biopsy specimens was investigated by immunohistochemical method.
p53 was well preserved in the samples of pleural effusion. p53 positive rate was 0%(0/27) ,33.3 %(2/6) and 54.2%(13/24) respectively in cytologically benign , suspicious but not conclusively malignant and malignant pleural effusion. Benign pleural effusion referred as tuberculous and other inflammatory effusion. p53 poisitive rate was 0%(0/23) ,14.3 %(1/7) and 35.9 % (14/39) in histologically benign , atypical proliferating and malignant bronchial biopsy sample. The cases with p53 immunoreactivity were proved to have lung cancer after follow-up in cytologically suspicious but not conclusively malignant pleural effusion and histologically atypical proliferating bronchial biopsy sample.
p53 immunostaining could be useful as a marker of malignancy in the cytologic examination of pleural effusion and bronchial biopsy sample. The presence of p53 immunoreactivity in these samples is strongly suggestive of malignancy , though its absence does not exclude neoplasms.
探讨p53免疫组化染色作为肺癌诊断中恶性肿瘤标志物的实用性。
采用免疫组化方法研究57例胸腔积液样本和69例支气管活检标本中p53的表达情况。
胸腔积液样本中p53保存良好。在细胞学检查为良性、可疑但不能确诊为恶性以及恶性胸腔积液中，p53阳性率分别为0%（0/27）、33.3%（2/6）和54.2%（13/24）。良性胸腔积液指结核性及其他炎性积液。在组织学检查为良性、非典型增生及恶性支气管活检样本中，p53阳性率分别为0%（0/23）、14.3%（1/7）和35.9%（14/39）。在细胞学检查可疑但不能确诊为恶性的胸腔积液以及组织学检查非典型增生的支气管活检样本中，经随访，p53免疫反应阳性的病例被证实患有肺癌。
p53免疫染色可作为胸腔积液和支气管活检样本细胞学检查中恶性肿瘤的标志物。这些样本中存在p53免疫反应强烈提示恶性肿瘤，但其缺失并不排除肿瘤的存在。
