Novosibirsk State University, Russia.
Cell Immunol. 2010;266(1):46-51. doi: 10.1016/j.cellimm.2010.08.011. Epub 2010 Sep 21.
A preparation of human genomic fragmented double-stranded DNA (dsDNA) was used as maturation stimulus in cultures of human dendritic cells (DCs) generated in compliance with the interferon protocol. Culturing of the DCs in medium with 5μg/ml of the DNA preparation was associated with a decrease in the relative proportion of CD14 + cells and an increase in that of CD83 + cells. These changes are markers of DC maturation. The efficiency with which the DNA preparation was able to elicit DC maturation was commensurate with that of lypopolysaccharide from bacterial cell, the standard inducer of DC maturation. Generated ex vivo, matured in the presence of the human DNA preparation, pulsed with tumor antigens mouse DCs were used as a vaccine in biological tests for its antitumor activity. The experimental results demonstrate that reinfusion of mature pulsed with tumor antigens DCs cause a statistically significant suppression of tumor graft growth.
采用人基因组片段化双链 DNA（dsDNA）制剂作为成熟刺激物，在符合干扰素方案的条件下培养人树突状细胞（DC）。在含有 5μg/ml DNA 制剂的培养基中培养 DC，与 CD14+细胞比例降低和 CD83+细胞比例增加相关。这些变化是 DC 成熟的标志物。DNA 制剂诱导 DC 成熟的效率与细菌细胞脂多糖相当，后者是 DC 成熟的标准诱导剂。在体外生成、在人 DNA 制剂存在下成熟、用肿瘤抗原脉冲的成熟 DC 作为疫苗用于其抗肿瘤活性的生物学试验。实验结果表明，成熟的肿瘤抗原脉冲 DC 的再输注导致肿瘤移植物生长的统计学显著抑制。
