人疱疹病毒6型编码的糖蛋白Q1基因对病毒生长至关重要。
Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth.
作者信息
Tang Huamin, Kawabata Akiko, Yoshida Mayumi, Oyaizu Hiroko, Maeki Takahiro, Yamanishi Koichi, Mori Yasuko
机构信息
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
出版信息
Virology. 2010 Nov 25;407(2):360-7. doi: 10.1016/j.virol.2010.08.018. Epub 2010 Sep 21.
Human herpesvirus 6 (HHV-6) glycoprotein Q1 (gQ1), a unique gene in HHV-6, forms a complex with glycoproteinH (gH) and gL, which is the viral ligand for its cellular receptor, CD46. However, whether gQ1 is essential for virus growth is unknown, because a system is lacking for making gene knockouts for HHV-6. Recently, bacterial artificial chromosome (BAC) and E. coli mutagenesis techniques have been applied to herpesvirus investigation. Here we successfully inserted the HHV-6A genome into a BAC, and obtained reconstituted infectious virus from the HHV-6A-containing BAC DNA. Using this system, we generated a gQ1 mutant virus genome, which failed to yield reconstituted infectious virus, whereas its revertant virus could be produced, indicating that the HHV-6 gQ1 gene is essential for virus growth. Therefore, we successfully applied BAC and E. coli mutagenesis techniques to the study of HHV-6, and discovered that HHV-6 gQ1 is an essential gene for virus growth.
人类疱疹病毒6型（HHV-6）糖蛋白Q1（gQ1）是HHV-6中的一个独特基因，它与糖蛋白H（gH）和gL形成复合物，而该复合物是其细胞受体CD46的病毒配体。然而，gQ1对于病毒生长是否必不可少尚不清楚，因为缺乏对HHV-6进行基因敲除的系统。最近，细菌人工染色体（BAC）和大肠杆菌诱变技术已应用于疱疹病毒研究。在此，我们成功地将HHV-6A基因组插入到BAC中，并从含HHV-6A的BAC DNA中获得了重组感染性病毒。利用该系统，我们构建了一个gQ1突变病毒基因组，该基因组无法产生重组感染性病毒，而其回复病毒则可以产生，这表明HHV-6 gQ1基因对于病毒生长至关重要。因此，我们成功地将BAC和大肠杆菌诱变技术应用于HHV-6的研究，并发现HHV-6 gQ1是病毒生长的必需基因。
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