Tang Huamin, Kawabata Akiko, Yoshida Mayumi, Oyaizu Hiroko, Maeki Takahiro, Yamanishi Koichi, Mori Yasuko
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Virology. 2010 Nov 25;407(2):360-7. doi: 10.1016/j.virol.2010.08.018. Epub 2010 Sep 21.
Human herpesvirus 6 (HHV-6) glycoprotein Q1 (gQ1), a unique gene in HHV-6, forms a complex with glycoproteinH (gH) and gL, which is the viral ligand for its cellular receptor, CD46. However, whether gQ1 is essential for virus growth is unknown, because a system is lacking for making gene knockouts for HHV-6. Recently, bacterial artificial chromosome (BAC) and E. coli mutagenesis techniques have been applied to herpesvirus investigation. Here we successfully inserted the HHV-6A genome into a BAC, and obtained reconstituted infectious virus from the HHV-6A-containing BAC DNA. Using this system, we generated a gQ1 mutant virus genome, which failed to yield reconstituted infectious virus, whereas its revertant virus could be produced, indicating that the HHV-6 gQ1 gene is essential for virus growth. Therefore, we successfully applied BAC and E. coli mutagenesis techniques to the study of HHV-6, and discovered that HHV-6 gQ1 is an essential gene for virus growth.
人类疱疹病毒6型（HHV-6）糖蛋白Q1（gQ1）是HHV-6中的一个独特基因，它与糖蛋白H（gH）和gL形成复合物，而该复合物是其细胞受体CD46的病毒配体。然而，gQ1对于病毒生长是否必不可少尚不清楚，因为缺乏对HHV-6进行基因敲除的系统。最近，细菌人工染色体（BAC）和大肠杆菌诱变技术已应用于疱疹病毒研究。在此，我们成功地将HHV-6A基因组插入到BAC中，并从含HHV-6A的BAC DNA中获得了重组感染性病毒。利用该系统，我们构建了一个gQ1突变病毒基因组，该基因组无法产生重组感染性病毒，而其回复病毒则可以产生，这表明HHV-6 gQ1基因对于病毒生长至关重要。因此，我们成功地将BAC和大肠杆菌诱变技术应用于HHV-6的研究，并发现HHV-6 gQ1是病毒生长的必需基因。
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