National Institute of Scientific Investigation, 331-1 Shinwol 7-Dong, Yang-Chun Gu, Seoul, South Korea.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Oct 15;878(28):2845-51. doi: 10.1016/j.jchromb.2010.08.040. Epub 2010 Sep 20.
In this paper, the incorporation of methamphetamine (MA) into rat hair was studied. The main purpose of this study was to investigate whether MA can be detected or positive hair results can be obtained in hair of rats administered a single dose of MA. The relationship between dose and frequency of administration and the concentrations of MA and its metabolite, amphetamine (AP), in rat hair were evaluated and the MA and AP concentrations in white and pigmented hair were compared. MA was administered to rats as follows: low dose (0.5mg/kg/day), medium dose (2mg/kg/day) and high dose (10mg/kg/day). The frequency of administration was one time per day for 1, 2, 3, 4, 5, 15 and 30 days. Hair and urine samples were collected from rats and analyzed by gas chromatography/mass spectrometry (GC/MS). MA could be identified in pigmented rat hair when MA was administered for 4 or more days at low daily dose and on day 1 following administration of medium and high daily doses. Positive results for MA were obtained from pigmented rat hair when MA was administered for 30 days at low daily dose, for 4 or more days at medium daily dose, or for 2 or more days at high daily dose. The concentrations of MA and AP found in rat hair were proportional to the dose and frequency of administration. The concentrations of MA and AP in pigmented rat hair were 2-10 times higher than those in white rat hair. The results of this study on the incorporation of MA into rat hair can serve as a model to better understand the incorporation of MA into human hair even though there are differences between animal models and human hair.
本研究旨在探讨单剂量 methamphetamine（MA）给药后大鼠毛发中是否能够检测到 MA 或呈阳性结果。评估了剂量和给药频率、MA 及其代谢物苯丙胺（AP）在大鼠毛发中的浓度之间的关系，并比较了白色和色素沉着毛发中 MA 和 AP 的浓度。MA 以如下方式给予大鼠：低剂量（0.5mg/kg/天）、中剂量（2mg/kg/天）和高剂量（10mg/kg/天）。给药频率为每天一次，持续 1、2、3、4、5、15 和 30 天。从大鼠中收集毛发和尿液样本，并通过气相色谱/质谱法（GC/MS）进行分析。当低日剂量给药 4 天或更长时间，以及中、高日剂量给药 1 天后，MA 可在色素沉着大鼠毛发中被识别。当低日剂量给药 30 天，中日剂量给药 4 天或更长时间，或高日剂量给药 2 天或更长时间时，MA 可从色素沉着大鼠毛发中获得阳性结果。MA 和 AP 在大鼠毛发中的浓度与剂量和给药频率成正比。色素沉着大鼠毛发中的 MA 和 AP 浓度比白色大鼠毛发中的浓度高 2-10 倍。本研究关于 MA 掺入大鼠毛发的结果可以作为一种模型，有助于更好地理解 MA 掺入人类毛发的情况，尽管动物模型和人类毛发之间存在差异。
