Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, P.O. Box 1052, E-41080 Seville, Spain.
Bioresour Technol. 2011 Jan;102(2):1338-43. doi: 10.1016/j.biortech.2010.08.112. Epub 2010 Sep 22.
The potential of the lipoxygenase from Gaeumannomyces graminis to remove lipophilic extractives from eucalypt and flax pulps was investigated. Pulp treatments were performed with the lipoxygenase both in the presence and absence of linoleic acid, and were followed by a peroxide bleaching stage. The main lipophilic extractives from eucalypt pulp such as conjugated and free sterols decreased up to 40% and 7%, respectively, by the lipoxygenase treatment in the presence of linoleic acid. Different degradation patterns were observed among the lipophilic compounds present in flax pulp, although a high removal of all the extractives classes, including alkanes (21-55%), fatty alcohols (42-61%), and free (16-55%) and glycosylated (45-71%) sterols, was attained in all the lipoxygenase treatments. Reactions of the lipoxygenase with model lipid mixtures were carried out to better understand the degradation patterns observed in pulps. Finally, pulp delignification by the lipoxygenase treatments was also evaluated.
研究了禾顶囊壳脂肪氧合酶从桉树和亚麻浆中去除亲脂性抽出物的潜力。在存在和不存在亚油酸的情况下，用脂肪氧合酶对纸浆进行处理，然后进行过氧化物漂白阶段。在存在亚油酸的情况下，脂肪氧合酶处理可使桉树纸浆中的主要亲脂性抽出物（如共轭和游离甾醇）分别降低 40%和 7%。在亚麻浆中存在的亲脂性化合物中观察到不同的降解模式，尽管在所有脂肪氧合酶处理中均实现了所有抽出物类别的高去除率，包括烷烃（21-55%）、脂肪醇（42-61%）以及游离（16-55%）和糖基化（45-71%）甾醇。进行了脂肪氧合酶与模型脂质混合物的反应，以更好地理解纸浆中观察到的降解模式。最后，还评估了脂肪氧合酶处理对纸浆脱木质素的作用。
