Maltsev V A
Institute of Immunology, USSR Ministry of Health, Moscow.
Immunol Lett. 1990 Dec;26(3):277-82. doi: 10.1016/0165-2478(90)90159-n.
Using the patch-clamp technique, membrane potential (MP) behaviour under different electrogenic stimuli for both resting and Con A-activated T lymphocytes was studied. The voltage-gated K+ channels stabilize MP at the level of their threshold of activation (-44 +/- 5 mV, n = 27). Upon depolarization of the cell membrane by external current pulse (in the current-clamp mode), MP can oscillate with a high amplitude of 20-50 mV. The amplitude depends on the number of K+ channels per cell and the external current value. After current pulse termination, MP did not return to its initial value, but continued to oscillate. However, the cells first activated by Con A did not display this triggering effect. Thus, the properties of T cell membrane revealed appear to be associated with the functional state of the cell.
采用膜片钳技术，研究了静息和刀豆球蛋白A（Con A）激活的T淋巴细胞在不同电刺激下的膜电位（MP）行为。电压门控钾通道将MP稳定在其激活阈值水平（-44±5 mV，n = 27）。通过外部电流脉冲使细胞膜去极化（电流钳模式）时，MP可产生20 - 50 mV的高振幅振荡。振幅取决于每个细胞钾通道的数量和外部电流值。电流脉冲终止后，MP不会回到其初始值，而是继续振荡。然而，最初由Con A激活的细胞未表现出这种触发效应。因此，所揭示的T细胞膜特性似乎与细胞的功能状态相关。
