Jones G L, Spencer L, Mollard R, Saul A
Queensland Institute of Medical Research, Herston, Australia.
Immunol Lett. 1990 Dec;26(3):285-90. doi: 10.1016/0165-2478(90)90161-i.
Thirty-five overlapping peptides from both conserved and variable parts of the N-terminal region of a malarial merozoite surface antigen (MSA2) were synthesised using solid phase chemistry. All peptides were synthesised with an added N-terminal cysteine and purified by reverse-phase HPLC to facilitate coupling to a carrier protein diphtheria toxoid (DT) using the hetero-bifunctional reagent maleimidocaproloxysuccinimide (MCS). Mice were immunised with these peptide-DT conjugates using Freund's complete adjuvant (FCA). The immune response of these mice was tested against peptide hapten, carrier protein (DT) and the linking region itself, using enzyme-linked immunoassay (ELISA). Although there was great variation in the immune response to each part of the immunogen construct, no significant correlation could be seen between each set of responses.
利用固相化学方法合成了疟原虫裂殖子表面抗原（MSA2）N端区域保守和可变部分的35个重叠肽段。所有肽段均在N端添加了半胱氨酸，并通过反相高效液相色谱法进行纯化，以便使用异双功能试剂马来酰亚胺己酰氧基琥珀酰亚胺（MCS）与载体蛋白白喉类毒素（DT）偶联。使用弗氏完全佐剂（FCA）对小鼠进行这些肽-DT偶联物免疫。使用酶联免疫吸附测定（ELISA）针对肽半抗原、载体蛋白（DT）和连接区域本身测试这些小鼠的免疫反应。尽管对免疫原构建体各部分的免疫反应存在很大差异，但每组反应之间未发现显著相关性。
