Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tüzoltó u 58, PO Box 95, Budapest 1450, Hungary.
Inflamm Res. 2011 Feb;60(2):163-8. doi: 10.1007/s00011-010-0250-0. Epub 2010 Sep 24.
Substance P (SP) elicits numerous potent neuroimmunomodulatory effects, increasing the release of tumor necrosis factor alpha (TNF-α). The study aimed to investigate immunoneural communication in experimentally-induced gastritis in rats.
SP-containing nerve fibers and lymphocytes and mast cells were counted in the mucosa of the stomachs of rats using double immunohistochemical and confocal laser microscopic methods, proving colocalization of SP and TNF-α in the lymphocytes and mast cells.
In controls, only the nerve fibers showed SP immunoreactivity (IR). However, in gastritis the number of SP-IR fibers and TNF-α IR lymphocytes and mast cells increased significantly (P < 0.001); SP-IR fibers were seen in close contact with lymphocytes and mast cells. Numerous lymphocytes (13.1%) and mast cells (10.8%) showed IR for both SP and TNF-α (colocalization) within the same cells.
SP release from nerve fibers, lymphocytes and mast cells together with TNF-α can enhance the development of gastric inflammation and participate in tissue damage in gastritis.
P 物质（SP）可引发多种强效的神经免疫调节作用，增加肿瘤坏死因子-α（TNF-α）的释放。本研究旨在探讨实验性大鼠胃炎中的免疫神经通讯。
采用双重免疫组织化学和共聚焦激光显微镜方法，对大鼠胃黏膜中的含 SP 神经纤维以及淋巴细胞和肥大细胞进行计数，证实了淋巴细胞和肥大细胞中 SP 和 TNF-α 的共定位。
在对照组中，仅神经纤维显示 SP 免疫反应性（IR）。然而，在胃炎中，SP-IR 纤维以及 TNF-α-IR 淋巴细胞和肥大细胞的数量显著增加（P < 0.001）；SP-IR 纤维与淋巴细胞和肥大细胞紧密接触。在同一细胞内，大量淋巴细胞（13.1%）和肥大细胞（10.8%）同时显示 SP 和 TNF-α 的 IR（共定位）。
神经纤维、淋巴细胞和肥大细胞中 SP 的释放以及 TNF-α 可以增强胃炎症的发展，并参与胃炎中的组织损伤。
