Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung city, Taiwan, 40227, People's Republic of China.
Biotechnol Lett. 2011 Jan;33(1):109-12. doi: 10.1007/s10529-010-0400-z. Epub 2010 Sep 24.
Conditions for the cell disruption of Xanthophyllomyces dendrorhous by using a bead beater have been optimized with respect to maximizing the recovery of carotenoids and transfructosylating enzymes in a one-step process. The optimal conditions for a one-step extraction of carotenoids and transfructosylating enzymes from Xanthophyllomyces dendrorhous were: 30 g dry cells/l being disintegrated with 0.5 mm diam. glass beads at 10°C for 15 min using a bead beater. More than 90% yeast disruption and, when extracted with hexane/ethyl acetate, 28.6 mg carotenoids/l in the organic solvent phase and 440 U transfructosylating enzyme activity/mg protein in the water phase were obtained.
已优化了使用珠磨机使束丝藻细胞破碎的条件，以在一步法中最大限度地回收类胡萝卜素和转果糖基酶。从束丝藻一步提取类胡萝卜素和转果糖基酶的最佳条件为：用珠磨机在 10°C 下用 0.5mm 直径的玻璃珠将 30g 干细胞/L 的细胞破碎 15min。超过 90%的酵母细胞被破坏，用正己烷/乙酸乙酯提取时，有机溶剂相中获得 28.6mg 类胡萝卜素/L，水相中获得 440U 转果糖基酶活性/mg 蛋白。
