Gogotov I N
Biochimie. 1978;60(3):267-75. doi: 10.1016/s0300-9084(78)80823-2.
Purple bacteria Rhodospirillum rubrum and Thiocapsa roseopersicina form two enzymes, hydrogenase and nitrogenase, which participate in hydrogen metabolism. H2 photoproduction in these bacteria is associated mainly or completely with the action of nitrogenase. The soluble and membrane-bound hydrogenases of T. roseopersicina have similar physicochemical properties (mol. weight, subunit composition, N-terminal amino acids, Fe2+ and S2- content, pl. Eo'). In comparison with other hydrogenases the enzyme from R. rubrum and T. roseopersicina evolve H2 with high rate from reduced cytochrome c3, but not from ferredoxins. H2 production and N2 fixation take place in the presence of NAD(P)H. NADP-reductase, ferredoxin and cytochrome c3 participate in this reaction. Possible relationships between hydrogenase-nitrogenase in the metabolism of molecular hydrogen are discussed.
紫色细菌红螺菌（Rhodospirillum rubrum）和玫瑰色硫囊菌（Thiocapsa roseopersicina）能形成两种参与氢代谢的酶，即氢化酶和固氮酶。这些细菌中的光致产氢主要或完全与固氮酶的作用相关。玫瑰色硫囊菌的可溶性氢化酶和膜结合氢化酶具有相似的物理化学性质（分子量、亚基组成、N端氨基酸、Fe2+和S2-含量、等电点、标准电极电位）。与其他氢化酶相比，来自红螺菌和玫瑰色硫囊菌的酶能以较高速率从还原型细胞色素c3而非铁氧化还原蛋白中产生H2。H2的产生和N2的固定在NAD(P)H存在的情况下发生。NADP-还原酶、铁氧化还原蛋白和细胞色素c3参与此反应。文中讨论了氢化酶-固氮酶在分子氢代谢中的可能关系。
