通过串联 cDNA 探针检测休眠马铃薯块茎中的马铃薯纺锤块茎类病毒（PSTV）

Detection of potato spindle tuber viroid (PSTV) in dormant potato tubers by concatameric cDNA probe.

作者信息

Zekanowski C, Wełnicki M, Skrzeczkowski J, Zagórski W

机构信息

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.

出版信息

J Virol Methods. 1990 Oct;30(1):127-30. doi: 10.1016/0166-0934(90)90050-p.

DOI:10.1016/0166-0934(90)90050-p

PMID:2086594

Abstract

The 32P-labelled concatameric insert cut out from a plasmid pSPAv6.2(+), containing 6.2 copies of a full-length PSTV, was used to detect PSTV in dormant potato tubers by dot-blot hybridisation assay. The concatameric insert probe was 4 times more sensitive than the monomeric one. This allowed the detection of 0.5 pg of viroid RNA. The sensitivity makes th eoligomeric cDNA probe a useful alternative to cDNA probes.

摘要

从质粒pSPAv6.2(+)中切下的含有6.2个全长马铃薯纺锤块茎类病毒（PSTV）拷贝的32P标记串联插入片段，用于通过斑点杂交试验检测休眠马铃薯块茎中的PSTV。串联插入探针的灵敏度比单体探针高4倍。这使得能够检测到0.5 pg的类病毒RNA。这种灵敏度使得寡聚cDNA探针成为cDNA探针的一种有用替代品。