Medical Faculty, University of Maribor, Slomškov trg 15, Maribor, Slovenia.
Arthropod Struct Dev. 2011 Jan;40(1):54-63. doi: 10.1016/j.asd.2010.09.002.
The cave crickets Troglophilus cavicola and Troglophilus neglectus are the most widely distributed European species of the family Rhaphidophoridae. Their life cycles span two years. They overwinter twice in caves in 4-6 months lasting diapause, T. cavicola in warmer microhabitats. In caves, older T. cavicola undergo sexual maturation, while T. neglectus do not. We hypothesized that the use of energy-supplying compounds and reserve proteins in the fat body is more extensive in T. cavicola than in T. neglectus. We analyzed the contents and morphology of lipid droplets, glycogen rosettes and protein granula at the beginning, the middle and the end of overwintering applying optic, TEM and biochemical methods. In all individuals, the fat body is composed of about 40 oval ribbons consisted of gradually changing adipocytes and urocytes. T. cavicola use glycogen continuously, and stop using lipids in the middle of overwintering, while this is inverse in T. neglectus. Till the middle of overwintering, all individuals exploit proteins, afterwards they are unevenly exploited. We found that the fat body is differently engaged in metabolism of both cave crickets during overwintering, supporting a more glycogen-dependent metabolism in T. cavicola, and a more lipid-dependent one in T. neglectus.
洞穴蟋蟀 Troglophilus cavicola 和 Troglophilus neglectus 是 Rhaphidophoridae 科在欧洲分布最广的物种。它们的生命周期跨越两年。它们在洞穴中经历两次越冬，持续 4-6 个月的休眠，T. cavicola 在较温暖的微生境中越冬。在洞穴中，较老的 T. cavicola 经历性成熟，而 T. neglectus 则不会。我们假设在 T. cavicola 中，脂肪体中提供能量的化合物和储备蛋白的利用比在 T. neglectus 中更为广泛。我们应用光学、TEM 和生化方法，在越冬开始、中期和结束时分析脂质滴、糖原玫瑰花和蛋白颗粒的含量和形态。在所有个体中，脂肪体由约 40 个椭圆形条带组成，由逐渐变化的脂肪细胞和尿细胞组成。T. cavicola 持续利用糖原，并在越冬中期停止利用脂质，而 T. neglectus 则相反。在越冬中期之前，所有个体都利用蛋白质，之后它们的利用程度不均匀。我们发现，在越冬期间，脂肪体对两种洞穴蟋蟀的代谢有不同的参与，支持 T. cavicola 中更依赖于糖原的代谢，而 T. neglectus 中更依赖于脂质的代谢。
