Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Clin Chim Acta. 2011 Jan 14;412(1-2):120-8. doi: 10.1016/j.cca.2010.09.015. Epub 2010 Sep 22.
Biomarkers are good potential tools for early cancer diagnosis. Here we have analyzed eight different pteridines in the urine samples of cancer patients and compared them with samples from healthy subjects. Pteridines are important cofactors in the process of cell metabolism, and they have recently become a focal point of cancer screening research because certain pteridine levels have been shown to reflect the presence of cancers.
We analyzed 8 pteridines; 6,7-dimethylpterin, 6-biopterin, d-(+)-neopterin, 6-hydroxymethylpterin, pterin, isoxanthopterin, xanthopterin and pterin-6-carboxylic acid using a house-built high-performance capillary electrophoresis with laser-induced fluorescence detection (HPCE-LIF). The levels of pteridines were reported as a ratio of pteridine to creatinine. Statistical hypothesis testing was conducted and P values were calculated to analyze the data.
Among the eight pteridines studied, 6-biopterin, 6-hydroxymethylpterin, pterin, xanthopterin, and isoxanthopterin levels were significantly higher in samples from cancer patients than in those from healthy subjects. Further, xanthopterin and isoxanthopterin levels were compared in breast cancer and lung cancer patients, but no significant difference was observed.
Some pteridine levels can be used as biomarkers for noninvasive diagnosis of cancer; however, more data is needed to support this hypothesis.
生物标志物是癌症早期诊断的潜在有效工具。在此，我们分析了癌症患者尿液样本中的 8 种不同的蝶呤，并将其与健康受试者的样本进行了比较。蝶呤是细胞代谢过程中的重要辅助因子，最近已成为癌症筛查研究的焦点，因为某些蝶呤水平反映了癌症的存在。
我们使用自行构建的高效毛细管电泳激光诱导荧光检测（HPCE-LIF）方法分析了 8 种蝶呤；6,7-二甲基蝶呤、6-生物蝶呤、d-（+）-新蝶呤、6-羟甲基蝶呤、蝶呤、异黄蝶呤、黄蝶呤和蝶呤-6-羧酸。蝶呤水平以蝶呤与肌酐的比值报告。进行了统计假设检验，并计算了 P 值以分析数据。
在所研究的 8 种蝶呤中，6-生物蝶呤、6-羟甲基蝶呤、蝶呤、黄蝶呤和异黄蝶呤在癌症患者的样本中明显高于健康受试者。此外，比较了乳腺癌和肺癌患者的黄蝶呤和异黄蝶呤水平，但未观察到显著差异。
一些蝶呤水平可以用作癌症非侵入性诊断的生物标志物；然而，需要更多的数据来支持这一假设。
